ABSTRACT Lead (Pb) is a toxic metal that can accumulate in the body and negatively impact human health. In this study, we prepared a highly sensitive and specific monoclonal antibody, 2C3, that can recognize Pb(II) with an IC50 and linear range of 0.5 ng/mL and 0.08–1.86 ng/mL, respectively. Additionally, based on this antibody we developed a fluorescent test strip, which can be used for both qualitative and quantitative detection of Pb(II) in brown rice with a detection range of 4.67–47.8 ng/mL. Therefore, the method we developed has high sensitivity suitable for the detection of Pb(II) in brown rice.

中文翻译：

---

用于检测铅的荧光定量条带检测方法的开发

摘要 铅 (Pb) 是一种有毒金属，可在体内蓄积并对人体健康产生负面影响。在本研究中，我们制备了一种高灵敏度和特异的单克隆抗体 2C3，它可以识别 Pb(II)，IC50 和线性范围分别为 0.5 ng/mL 和 0.08–1.86 ng/mL。此外，基于该抗体，我们开发了一种荧光试纸，可用于糙米中 Pb(II) 的定性和定量检测，检测范围为 4.67–47.8 ng/mL。因此，我们开发的方法具有高灵敏度，适用于糙米中 Pb(II) 的检测。