ABSTRACT In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold immunochromatographic assay were developed to detect the furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). The detection of AMOZ was based on the AMOZ derivative 3-([2-nitrophenyl] methyleneamino)-5-morpholinomethyl-2-oxazolidinone (2-NPAMOZ). The monoclonal anti-2-NPAMOZ antibody 4G11, was prepared and exhibited a 50%-inhibitory concentration (IC50) of 0.049 ng/mL. Its cross-reactivity with structurally related analogues was less than 3%. This immunochromatographic assay was assembled for the rapid screening of AMOZ in catfish samples. The strip assay had a cut-off value of 0.1 μg/kg and a limit of detection (LOD) of 0.009 μg/kg. Analysis of AMOZ in catfish indicated that the results from the strip assay were highly comparable to results obtained using ic-ELISA and LC-MS/MS. Therefore, the colloidal gold (CG) immunoassay is a sensitive screening method for the detection of AMOZ residues in catfish samples.

中文翻译：

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用于快速检测鲶鱼中 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) 的金免疫层析试纸条的研制

摘要 在本研究中，开发了一种间接竞争性酶联免疫吸附试验 (ic-ELISA) 和金免疫层析试验来检测呋喃他酮代谢物 3-氨基-5-吗啉甲基-2-恶唑烷酮 (AMOZ)。AMOZ 的检测基于 AMOZ 衍生物 3-([2-硝基苯基] 亚甲氨基)-5-吗啉甲基-2-恶唑烷酮 (2-NPAMOZ)。制备了单克隆抗 2-NPAMOZ 抗体 4G11，其 50% 抑制浓度 (IC50) 为 0.049 ng/mL。其与结构相关类似物的交叉反应性小于 3%。该免疫层析分析用于快速筛选鲶鱼样品中的 AMOZ。条带检测的临界值为 0.1 μg/kg，检测限 (LOD) 为 0.009 μg/kg。鲶鱼中 AMOZ 的分析表明，条带检测的结果与使用 ic-ELISA 和 LC-MS/MS 获得的结果具有高度可比性。因此，胶体金 (CG) 免疫测定法是检测鲶鱼样品中 AMOZ 残留的灵敏筛选方法。