The well-studied quorum sensing (QS) mechanism has established a complex knowledge system of how microorganisms behave collectively in natural ecosystems, which contributes to bridging the gap between the ecological functions of microbial communities and the molecular mechanisms of cell-to-cell communication. In particular, the ability of agrochemical degradation has been one most attractive potential of functional bacteria, but the interaction and mutual effects of intracellular degradation and intraspecific behavior remained unclear. In this study, we establish a connection between QS regulation and biodegradation by harnessing the previously isolated Bacillus subtilis BSF01 as a template which degrades various pyrethroids. First, we characterize the genetic and transcriptional basis of comA-involved QS system in B. subtilis BSF01 since the ComQXPA circuit coordinates group behaviors in B. subtilis isolates. Second, the genetic and transcriptional details of pyrethroid-degrading carboxylesterase CesB are defined, and its catalytic capacity is evaluated under different conditions. More importantly, we adopt DNA pull-down and yeast one-hybrid techniques to reveal that the enzymatic degradation of pyrethroids is initiated through QS signal regulator ComA binding to carboxylesterase gene cesB, highlighting the synergistic effect of QS regulation and pyrethroid degradation in B. subtilis BSF01. Taken together, the elucidated mechanism provides novel details on the intercellular response of functional bacteria against xenobiotic exposure, which opens up possibilities to facilitate the in-situ contaminant bioremediation via combining the QS-mediated strategies.

中文翻译：

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枯草芽孢杆菌 BSF01 的群体感应调节的羧酸酯酶协同降解拟除虫菊酯

经过充分研究的群体感应 (QS) 机制建立了一个关于微生物如何在自然生态系统中集体行为的复杂知识系统，这有助于弥合微生物群落的生态功能与细胞间通讯的分子机制之间的差距。尤其是农药降解能力一直是功能细菌最具吸引力的潜力之一，但细胞内降解和种内行为的相互作用和相互影响尚不清楚。在这项研究中，我们通过利用先前分离的枯草芽孢杆菌 BSF01 作为降解各种拟除虫菊酯的模板，建立了 QS 调节和生物降解之间的联系。首先，我们描述了 B 中涉及 comA 的 QS 系统的遗传和转录基础。subtilis BSF01，因为 ComQXPA 电路协调 B. subtilis 分离株中的群体行为。其次，定义了拟除虫菊酯降解羧酸酯酶 CesB 的遗传和转录细节，并在不同条件下评估了其催化能力。更重要的是，我们采用 DNA pull-down 和酵母单杂交技术揭示了拟除虫菊酯的酶促降解是通过 QS 信号调节剂 ComA 与羧酸酯酶基因 cesB 结合引发的，突出了枯草芽孢杆菌中 QS 调控和拟除虫菊酯降解的协同作用BSF01。总之，阐明的机制提供了关于功能性细菌对异生物质暴露的细胞间反应的新细节，这开辟了通过结合 QS 介导的策略促进原位污染物生物修复的可能性。