Post-antibiotic effect (PAE) is the continued suppression of bacterial growth following a limited exposure to an antimicrobial agent. The presence of PAE needs consequential consideration in designing antibiotic dosage regimens. To understand the behavior of bacteria, PAE provides information on how long antibiotics are applied to the bacteria. Conventional methods of measuring PAE depend on population detection and have limitations for understanding the individual behavior of bacteria. To observe the PAE, we utilized an imaging technique with the use of microscopy. Here, we discuss the microscopic image analysis system we used to study the PAE at a single-colony level. The size and number of colonies of bacteria were measured prior to and following antibiotic removal. We could count a single colony, see the development of the settlement prior to and following exposure of antibiotics and track the colony by microscopy according to the incubation time and the image processed by our own image processing program. The PAE of antibiotics was quantified by comparing bacteria size and number based on their exposure time. In our study, we discovered that the longer exposure of antibiotics causes the bacteria to be suppressed—even after washing the antibiotics from the solution. This finding suggests that microscopic imaging detection provides a new method for understanding PAE. In addition, the behavior of the cell in response to drugs and chemicals and their removal can be examined with the use of single colony analysis.

中文翻译：

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基于显微成像分析的氨苄青霉素和左氧氟沙星对大肠杆菌和金黄色葡萄球菌的抗生素作用。

抗生素后作用（PAE）是在有限接触抗菌剂后继续抑制细菌生长。在设计抗生素剂量方案时，需要考虑PAE的存在。为了了解细菌的行为，PAE提供了有关将抗生素应用到细菌上的时间的信息。测量PAE的常规方法取决于种群检测，并且在理解细菌的个体行为方面有局限性。为了观察PAE，我们利用成像技术和显微镜技术。在这里，我们讨论了用于在单菌落水平上研究PAE的显微图像分析系统。在去除抗生素之前和之后测量细菌菌落的大小和数量。我们可以算一个殖民地 观察抗生素暴露前后的定居情况，并根据孵育时间和由我们自己的图像处理程序处理的图像，通过显微镜观察菌落。通过根据细菌的暴露时间比较细菌的大小和数量来量化抗生素的PAE。在我们的研究中，我们发现，长时间暴露于抗生素会使细菌受到抑制，即使从溶液中清洗了抗生素也是如此。这一发现表明，显微成像检测提供了一种了解PAE的新方法。另外，可以通过单菌落分析来检查细胞对药物和化学物质的反应及其去除的行为。