This study investigated the efficacy of Lippia grata essential oil (EO) against monogeneans of Colossoma macropomum and effects on the haematology after immersion baths. In the in vitro assays, the efficacy of 100, 250, 350 and 700 mg/L of L. grata EO and two controls were tested, of which one control was with the cultivation tank water and the other was with the tank water and 70% ethyl alcohol. Composition of majority bioactive compounds in the EO was carvacrol (48.12%), p‐cymene (24.39%) and γ‐terpinene (2.49%). Anacanthorus spathulatus, Notozothecium janauachensis, Mymarothecium boegeri and Linguadactyloides brinkmanni obtained of fish gills showed 100% immobilization in the in vitro assays when exposed to 700 and 350 mg/L after 30 min and 2 hr respectively. Fish showed tolerance to 700 mg/L of the EO, which was used in therapeutic in three consecutive baths, which caused lamellar hyperplasia in C. macropomum gills. The 700 mg/L of the EO in the therapeutic baths showed an efficacy of 95.1% against the monogeneans and caused decrease in haemoglobin, whereas control fish showed a reduction in mean corpuscular haemoglobin concentration. Results indicated that 700 mg/L of L. grata EO is a safe and effective concentration to treat C. macropomum infected with monogeneans.

中文翻译：

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Lippia grata（Verbenaceae）的精油可有效控制巨大巨g（Colossoma macropomum gills）（一种来自亚马逊的大型锯齿鱼类）中的单基因感染。

本研究调查了Lippia grata精油（EO）对抗巨大巨像的单基因的功效以及对浸浴后血液学的影响。在体外测定中，100、250、350和700 mg / L L的功效。测试了 grata EO和两个对照，其中一个对照是培养槽水，另一个是培养槽水和70％乙醇。EO中大多数生物活性化合物的成分为香芹酚（48.12％），对伞花烯（24.39％）和γ-萜品烯（2.49％）。Anacanthorus spathulatus，Jonauothecium janauachensis，Boegeri霉菌和Bingmanni当分别在30分钟和2小时后暴露于700和350 mg / L的体外试验中，获得的鱼ill显示100％固定。鱼对EO的耐受性为700 mg / L，连续三个水浴用于治疗，导致C层状增生。 大型po。在治疗浴中700 mg / L的EO对单基因细菌显示95.1％的功效，导致血红蛋白下降，而对照鱼的平均红细胞血红蛋白浓度下降。结果表明的那700 mg / L的大号。 grata EO是治疗C的安全有效的浓度。 被单基因感染的巨乳。