Endothelial microvesicles (EMVs) could reflect the status of endothelial cells (ECs) which are involved in the pathogenesis of ischemic stroke (IS). MiR‐155 could regulate EC functions. However, their roles in IS remain unclear. This study aimed to investigate the levels of plasma EMVs and EMVs carrying miRNA‐155 (EMVs‐miR‐155) in IS patients to explore their potential roles as biomarkers. Ninety‐three IS patients and 70 controls were recruited in this study. The levels of circulating EMVs and EMVs‐miR‐155 were detected by fluorescence nanoparticle tracking analysis and quantitative real‐time PCR, respectively. The correlations between level of EMVs/EMVs‐miR‐155 and the onset time, severity, infarct volume, and subtypes of IS were analyzed. The severity and infarct volume were assessed by NIHSS and magnetic resonance imaging, respectively. Multivariate logistic regression analysis was used to investigate the risk factors of IS. The ROC curve and area under ROC curve (AUC) of EMVs and EMVs‐miR‐155 were determined. The levels of plasma EMVs and EMVs‐miR‐155 were increased significantly in acute and subacute stages of IS and remained unchanged in chronic stage, and were positively related to the infarct volume and NIHSS scores and were associated with large artery atherosclerosis and cardioembolism subtypes defined by Trial of Org 10 172 in acute stroke treatment (TOAST) classification. Multivariate logistic regression analysis demonstrated that plasma EMVs and EMVs‐miR‐155 were significant and independent risk factors of IS and their AUC were 0.778 and 0.851, respectively, and increased to 0.892 after combination. Our study suggests that plasma EMVs and EMVs‐miR‐155 are promising biomarkers for IS. The diagnostic value of EMVs‐miR‐155 is higher and their combination is the best.

中文翻译：

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血浆内皮微泡及其携带的 miRNA-155 作为缺血性中风的生物标志物。

内皮微泡 (EMV) 可以反映参与缺血性中风 (IS) 发病机制的内皮细胞 (EC) 的状态。MiR-155 可以调节 EC 功能。然而，他们在IS中的作用仍不清楚。本研究旨在调查 IS 患者血浆 EMV 和携带 miRNA-155 的 EMV（EMVs-miR-155）的水平，以探索它们作为生物标志物的潜在作用。本研究招募了 93 名 IS 患者和 70 名对照者。分别通过荧光纳米粒子跟踪分析和定量实时 PCR 检测循环 EMV 和 EMV-miR-155 的水平。分析了 EMVs/EMVs-miR-155 水平与 IS 的发病时间、严重程度、梗死体积和亚型之间的相关性。严重程度和梗死体积分别通过 NIHSS 和磁共振成像评估。多因素logistic回归分析用于调查IS的危险因素。测定了EMVs和EMVs-miR-155的ROC曲线和ROC曲线下面积（AUC）。血浆 EMVs 和 EMVs-miR-155 水平在 IS 的急性和亚急性期显着升高，在慢性期保持不变，与梗死体积和 NIHSS 评分呈正相关，并与大动脉粥样硬化和定义的心脏栓塞亚型相关通过 Org 10 172 在急性卒中治疗 (TOAST) 分类中的试验。多因素logistic回归分析表明，血浆EMVs和EMVs-miR-155是IS的显着独立危险因素，其AUC分别为0.778和0.851，联合用药后增加至0.892。我们的研究表明，血浆 EMV 和 EMV-miR-155 是有希望的 IS 生物标志物。EMVs-miR-155的诊断价值较高，两者结合效果最好。