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Protein L chromatography: A useful tool for monitoring/separating homodimers during the purification of IgG-like asymmetric bispecific antibodies.
Protein Expression and Purification ( IF 1.6 ) Pub Date : 2020-07-29 , DOI: 10.1016/j.pep.2020.105711
Xiujuan Chen 1 , Ying Wang 1 , Ying Wang 1 , Yifeng Li 1
Affiliation  

Asymmetric IgG-like bispecific antibodies (bsAbs) are normally derived from two parental mAbs with different origin. Despite the implementation of heterodimerization-promoting strategy in the design, homodimerization can still occur at a low level during the recombinant production of these molecules. In general, monitoring and removal of homodimers pose great challenges to analytical and purification teams, respectively, as these byproducts share high similarity in physicochemical properties with the target heterodimeric bsAb. Protein L is a bacterial surface protein that binds to the variable region of kappa light chain without interfering with the antigen binding site. In this work, we first showed that different antibodies bind Protein L-conjugated resin with varied strength, and then based on this observation we further demonstrated that Protein L chromatography can be a useful tool for monitoring/separating homodimers during the purification of asymmetric bsAbs.



中文翻译:

蛋白质L色谱法:一种在IgG样不对称双特异性抗体纯化过程中监测/分离同型二聚体的有用工具。

不对称IgG样双特异性抗体(bsAb)通常衍生自两个来源不同的亲本mAb。尽管在设计中实施了促进异二聚化的策略,但在重组生产这些分子的过程中,同二聚化仍可能以低水平发生。通常,同二聚体的监测和去除分别对分析和纯化团队构成巨大挑战,因为这些副产物在理化性质上与目标异二聚体bsAb具有高度相似性。蛋白L是一种细菌表面蛋白,可与κ轻链可变区结合而不会干扰抗原结合位点。在这项工作中,我们首先证明了不同的抗体以不同的强度结合蛋白L共轭树脂,

更新日期:2020-07-29
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