Abstract

We have studied manganese-containing calcium phosphates differing in manganese content and phase composition. At a manganese content of 0.15 wt %, the whitlockite content is ~90 wt % and the calcium pyrophosphate content is ~10 wt %. Increasing the manganese content to 1.49 wt % reduces the whitlockite content to ~70 wt % and increases the calcium pyrophosphate content to ~30 wt %. IR spectroscopy results show that the samples calcined at 400°C have the apatite structure, whereas raising the calcination temperature to 900°C leads to the formation of the whitlockite structure and calcium pyrophosphate. Active sintering begins in the range 920–1050°C. Raising the firing temperature to 1200°C leads to the formation of a densely sintered structure, with melted regions and an average grain size from 15 to 25 μm. With increasing manganese content, the grain size of the ceramics decreases. Our results on cytotoxic properties demonstrate that the samples are not cytotoxic and maintain cell proliferation and spreading. The cytotoxicity of the samples is insensitive to the calcium pyrophosphate and manganese concentrations. The powders and ceramics prepared in this study can be used as key components of novel materials for bone tissue engineering.

中文翻译：

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含锰磷酸钙材料的合成与性能

摘要

我们研究了锰含量和相组成不同的含锰磷酸钙。锰含量为0.15重量％时，辉锰矿含量为〜90重量％，焦磷酸钙含量为〜10重量％。锰含量增加到1.49 wt％会使辉锰矿含量减少到〜70 wt％，焦磷酸钙含量增加到〜30 wt％。红外光谱结果表明，在400°C煅烧的样品具有磷灰石结构，而将煅烧温度提高到900°C则导致形成了辉锰矿结构和焦磷酸钙。主动烧结在920–1050°C的范围内开始。将烧成温度提高到1200℃导致形成致密的烧结结构，其具有熔化的区域和15至25μm的平均晶粒尺寸。随着锰含量的增加，陶瓷的晶粒尺寸减小。我们关于细胞毒性特性的结果表明，这些样品没有细胞毒性，并且可以维持细胞增殖和扩散。样品的细胞毒性对焦磷酸钙和锰浓度不敏感。在这项研究中制备的粉末和陶瓷可用作骨组织工程新材料的关键成分。