Salmonella is a gram-negative bacterium belonging to the Enterobacteriaceae family. One of the major known serotypes of this bacterium is Salmonella typhi. This pathogen is the main cause of diseases in humans and livestock. It is transmitted from animals to humans through the consumption of contaminated food. At the moment, one way to prevent this illness is using the live attenuated Ty21a vaccine. To eliminate the side effects of the vaccine and improve its safety, it is important to use epitope subunit vaccines. In this study, a wide range of accurate online servers have been used to predict the epitopes of OmpA, OmpC and OmpF antigens of Salmonella typhi. The T-cell (MHCI and MHCII) and B-cell epitopes share a degree of overlap between these tools. The highest scores were chosen for the study. Further analysis of their antigenicity and resistance to digestive compounds was performed by VaxiJen Servers and Protein Digest Servers. Eventually, the resultant epitopes that had desired antigenicity (≥ 1) and showed the highest resistance to digestive compounds were selected. Using these epitopes (MHCI, MHCII and B cell) two different structures (antigenic structure and digestion-resistant structure) were designed by rigid linkers. These structures were based on the highest antigenicity ranking of epitopes and the highest resistance of epitopes to digestive compounds, respectively. Given that this pathogen is primarily transmitted through contaminated foods, oral vaccines are essential in this regard. Therefore, these structures can be considered as suitable candidates for subunit vaccines.

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计算基于不同抗原决定簇的伤寒沙门氏菌疫苗的设计

沙门氏菌是属于肠杆菌科的革兰氏阴性细菌。该细菌的主要已知血清型之一是伤寒沙门氏菌。该病原体是人类和牲畜疾病的主要原因。它通过食用被污染的食物从动物传播给人类。目前，预防这种疾病的一种方法是使用减毒活Ty21a疫苗。为了消除疫苗的副作用并提高其安全性，重要的是使用表位亚基疫苗。在这项研究中，广泛的准确的在线服务器已被用于预测伤寒沙门氏菌的OmpA，OmpC和OmpF抗原的表位。T细胞（MHCI和MHCII）和B细胞表位在这些工具之间共享一定程度的重叠。选择最高分数进行研究。VaxiJen Servers和Protein Digest Servers对其抗原性和对消化化合物的抗性进行了进一步分析。最终，选择具有所需抗原性（≥1）并显示出对消化化合物的最高抗性的所得表位。使用这些表位（MHCI，MHCII和B细胞），通过刚性接头设计了两种不同的结构（抗原结构和抗消化结构）。这些结构分别基于表位的最高抗原性等级和表位对消化化合物的最高抗性。由于这种病原体主要是通过受污染的食物传播的，因此口服疫苗在这方面至关重要。