Abstract

The spatial organization of the genome is controlled by a special class of architectural proteins, in particular proteins containing ZAD domains that are able to form homo- and heterodimers. These domains are unique for arthropods and are present at the N-terminus of many proteins containing C2H2-type zinc fingers. Despite a growing interest in the characterization of architectural proteins, little is known about their structures (structures of their functional domains), which could shed light on the mechanisms of regulatory processes of the establishment and maintenance of the spatial organization of chromatin domains. Only two structures of ZAD domains are known. Here we report the crystallization and preliminary X-ray diffraction analysis of the previously unknown ZAD domain of the Serendipity-d protein from Drosophila melanogaster.

中文翻译：

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果蝇Serendipity-d蛋白的ZAD结构域的结晶和X射线衍射分析

摘要

基因组的空间组织受一类特殊的建筑蛋白控制，特别是含有能够形成同二聚体和异二聚体的ZAD域的蛋白质。这些域对于节肢动物而言是唯一的，并且存在于许多含有C2H2型锌指的蛋白质的N末端。尽管人们越来越关注建筑蛋白的表征，但对其结构（其功能域的结构）了解甚少，这可能有助于阐明染色质域空间组织的建立和维持的调控过程机制。ZAD域只有两个结构是已知的。在这里，我们报告了来自未知的Serendipity-d蛋白的未知ZAD域的结晶和初步X射线衍射分析果蝇。