Human infections by pleosporalean fungi (class Dothideomycetes, phylum Ascomycota) are rarely reported. Because their identification is challenging using morphological characterization, several phylogenetic markers must be sequenced for an accurate identification and taxonomical placement of the isolates. Three isolates of clinical origin were phenotypically characterized, but due to the absence of relevant morphological traits, D1-D2 domains of the 28S nrRNA gene (LSU), the internal transcribed spacer region (ITS) of the nrRNA, and fragments of the RNA polymerase II subunit 2 (rpb2) and translation elongation factor 1-alpha (tef1) genes were sequenced to allow a phylogenetic analysis that would solve their phylogenetic placement. That analysis revealed that these isolates did not match any previously known pleosporalean genera, and they are proposed here as the new fungal genus, Gambiomyces. Unfortunately, the isolates remained sterile, which, consequently, made the morphological description of the reproductive structures impossible. Future studies should try to understand the behaviour of this fungus in nature as well as its characteristics as an opportunistic fungal pathogen. Molecular identification is becoming an essential tool for proper identification of Dothideomycetes of clinical origin.

中文翻译：

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从浅层到深层的人类临床标本中分离出的一种新的多孢子菌属真菌。

人类感染多孢子菌（Dothideomycetes，子囊菌门）的报道很少。由于使用形态学特征进行鉴定具有挑战性，因此必须对几个系统发育标记进行测序，以便准确鉴定和分类定位分离物。三个临床来源的分离株具有表型特征，但由于缺乏相关的形态特征、28S nrRNA 基因 (LSU) 的 D1-D2 结构域、nrRNA 的内部转录间隔区 (ITS) 和 RNA 聚合酶的片段II 亚基 2 ( rpb 2) 和翻译延伸因子 1-alpha ( tef1) 对基因进行测序以进行系统发育分析，从而解决它们的系统发育位置。该分析表明，这些分离株与任何先前已知的 pleosporalean 属都不匹配，因此在此建议将它们作为新的真菌属Gambiomyces。不幸的是，分离物仍然不育，因此无法对生殖结构进行形态学描述。未来的研究应该尝试了解这种真菌在自然界中的行为及其作为机会性真菌病原体的特征。分子鉴定正成为正确鉴定临床来源的Dothideomycetes的重要工具。