Abstract Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) (genus: Alphabaculovirus, incertae sedis: Baculoviridae) has been used to control Helicoverpa armigera (Hübner). A reproducible and susceptible cell line was prepared from the hemocytes of Ephestia kuehniella in Grace and Ex-Cell 420 media. The population doubling time of these cloned cell cultures during the logarithmic phase were about 2.3 and 3.7 d for Ex-Cell 420 and Grace's media, respectively. When 60% confluence occurred, cells were infected by viral inoculums. All biochemical compounds were significantly changed relevant to cellular metabolism due to HearNPV infection. In order to improve its stability, two polymer formulations were used, i.e., formulation A (sodium alginate, gelatin, starch, and molasses) and formulation B (cottonseed kernel extract, Bran, glycerol, boric acid, egg white, and sugar). Formulant A provided high photostability by exhibiting 83.2 ± 3% efficacy and 88.66 ± 2.1% original activities remaining after 72 h UV exposure. Percentage original activity remaining of unformulated HearNPV and formulated mixture of B was 38.66 ± 2.6% and 9.33 ± 1.3%, respectively, after 72 h UV-irradiation. The virulence of the HearNPV proliferated from the Ex-Cell medium was similar to the virulence of wild-type HearNPV with LC50 of 7.7×105 OBs/ml. Formulant A, revealed only 20.0 ± 1% reduction in efficacy while the unformulated virus and formulant B faced a reduction of 90.0 ± 3% and 64.0 ± 2% after 72 h of UVA irradiation. Formulant A thus showed a high potential to protect HearNPVs microparticles against UV-inactivation suggesting a new platform for more efficient biological-management of cotton bollworm (specific name Helicoverpa armigera, genus: Helicoverpa, Lepidoptera: Noctuidae) in vivo.

中文翻译：

---

开发 Ephestia kuehniella 血细胞系以评估微囊化棉铃虫核多角体病毒对棉铃虫（鳞翅目：夜蛾科）幼虫的生物杀虫潜力

摘要棉铃虫核多角体病毒（HearNPV）（属：Alphabaculovirus，incertae sedis：Baculoviridae）已被用于控制棉铃虫（Hübner）。在 Grace 和 Ex-Cell 420 培养基中，从 Ephestia kuehniella 的血细胞中制备了可重复且易感的细胞系。对于 Ex-Cell 420 和 Grace 培养基，这些克隆细胞培养物在对数期的群体倍增时间分别约为 2.3 天和 3.7 天。当发生 60% 汇合时，细胞被病毒接种物感染。由于 HearNPV 感染，所有与细胞代谢相关的生化化合物都发生了显着变化。为了提高其稳定性，使用了两种聚合物配方，即配方 A（海藻酸钠、明胶、淀粉和糖蜜）和配方 B（棉籽仁提取物、麸皮、甘油、硼酸、蛋清和糖）。配方 A 通过在紫外线照射 72 小时后表现出 83.2 ± 3% 的功效和 88.66 ± 2.1% 的原始活性来提供高光稳定性。在紫外线照射 72 小时后，未配制的 HearNPV 和配制的 B 混合物的原始活性百分比分别为 38.66 ± 2.6% 和 9.33 ± 1.3%。从 Ex-Cell 培养基增殖的 HearNPV 的毒力与野生型 HearNPV 的毒力相似，LC50 为 7.7×105 OBs/ml。配方 A 仅显示 20.0 ± 1% 的功效降低，而未配制的病毒和配方 B 在 UVA 照射 72 小时后面临 90.0 ± 3% 和 64.0 ± 2% 的功效降低。