ABSTRACT Ochratoxin A (OTA) is a highly toxic potential contaminant of most agricultural products, and OTA is difficult to remove in general food production processes. In this study, we immobilized polyvinyl pyrrolidone-coated carboxypeptidase A (CPA) into zeolitic imidazolate framework material to improve the ability for OTA degradation. In this method, carboxypeptidase A was dispersed well in the material and maintained its stability and ability to degrade OTA, under complex matrix conditions with better repeated use performance than free carboxypeptidase A. The substrate conversion rate of CPA increased 3 times after immobilization. After repeated use of 10 cycles, the composite enzyme still retained 71.37% of its initial activity. The degradation rate of OTA was 30.69% higher than that of free CPA. This method may play an important role in the degradation of mycotoxins.

中文翻译：

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用沸石咪唑酯骨架固定羧肽酶 A 以增强赭曲霉毒素 A 的降解能力

摘要赭曲霉毒素 A (OTA) 是大多数农产品的剧毒潜在污染物，OTA 在一般食品生产过程中难以去除。在这项研究中，我们将聚乙烯吡咯烷酮包被的羧肽酶 A (CPA) 固定到沸石咪唑酯骨架材料中，以提高降解 OTA 的能力。在该方法中，羧肽酶A在材料中分散良好，并保持其稳定性和降解OTA的能力，在复杂的基质条件下具有比游离羧肽酶A更好的重复使用性能。固定化后CPA的底物转化率提高了3倍。重复使用10个循环后，复合酶仍保留其初始活性的71.37%。OTA的降解率比游离CPA高30.69%。