ABSTRACT In this study we developed a rapid and simple assay to detect RFP in fish using an immunochromatographic assay. We produced a sensitive and selective monoclonal antibody against RFP with a half-maximal inhibitory concentration (IC50) of 10.7 ng/mL. Fish samples were extracted with ethyl acetate, then centrifuged at 8000 rpm for 10 min, and concentrated by drying over nitrogen. We found under optimal conditions, that the RFP cut-off limits for the test strip was 10 µg/mL in both 0.01 M phosphate buffered saline and fish samples, and each test could be completed within 5 min. Therefore, immunochromatographic test can be used as a reliable, rapid, and sensitive method for the detection of RFP in fish.

中文翻译：

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使用免疫层析试纸条快速检测鱼中的利福平

摘要 在这项研究中，我们开发了一种快速简单的检测方法，使用免疫色谱法检测鱼中的 RFP。我们生产了一种针对 RFP 的灵敏且选择性的单克隆抗体，其半数抑制浓度 (IC50) 为 10.7 ng/mL。用乙酸乙酯提取鱼样品，然后以 8000 rpm 离心 10 分钟，并通过氮气干燥浓缩。我们发现在最佳条件下，0.01 M 磷酸盐缓冲盐水和鱼样品中测试条的 RFP 截止限值均为 10 µg/mL，并且每个测试都可以在 5 分钟内完成。因此，免疫层析试验可作为一种可靠、快速、灵敏的方法检测鱼类中的RFP。