In this study, Chlorococcum sp. was investigated for its cholinesterase inhibitory potentials and antioxidant activity. The algal sample was cultivated, harvested, and extracted sequentially using n‐hexane, dichloromethane, and ethanol. The extracts were characterized using Fourier transmission infra‐red (FTIR) and Gas Chromatography‐Mass Spectrometry. The metal chelating, radical scavenging activities, as well as anticholinesterase potentials of the algal extract, was also investigated. FTIR characterization of the microalgal biomass revealed the presence of phenolic compounds, alkaloids, polysaccharides, and fatty acids. The extracts showed the presence of phytol, neophytadiene, butylated hydroxyl toluene, and 3‐tert‐butyl‐4‐hydroxyanisole. The ethanol extract showed the highest DPPH (IC₅₀ = 147.40 µg/ml) and OH (IC₅₀ = 493.90 µg/ml) radical scavenging and metal chelating (IC₅₀ = 83.25 µg/ml) activities. Similarly, the ethanol extract (IC₅₀ = 13.83 µg/ml) exhibited the highest acetylcholinesterase inhibitory activity, while the dichloromethane extract showed the highest butyrylcholinesterase inhibitory activity. All the extracts exhibited antioxidant properties and inhibitory effects against butyrylcholinesterase and acetylcholinesterase; however, ethanol extracts showed better activity.

中文翻译：

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Chlorococcum sp. 的胆碱酯酶抑制活性、抗氧化特性和植物化学成分。提取物

在这项研究中，绿球藻。研究了其胆碱酯酶抑制潜力和抗氧化活性。使用正己烷、二氯甲烷和乙醇依次培养、收获和提取藻类样品。使用傅里叶透射红外 (FTIR) 和气相色谱-质谱法对提取物进行表征。还研究了藻类提取物的金属螯合、自由基清除活性以及抗胆碱酯酶潜力。微藻生物质的 FTIR 表征揭示了酚类化合物、生物碱、多糖和脂肪酸的存在。提取物显示存在植醇、新植二烯、丁基羟基甲苯和 3-叔丁基-4-羟基苯甲醚。乙醇提取物显示出最高的 DPPH (IC ₅₀ = 147.40 µg/ml) 和 OH (IC ₅₀  = 493.90 µg/ml) 自由基清除和金属螯合 (IC ₅₀  = 83.25 µg/ml) 活性。类似地，乙醇提取物 (IC ₅₀  = 13.83 µg/ml) 表现出最高的乙酰胆碱酯酶抑制活性，而二氯甲烷提取物表现出最高的丁酰胆碱酯酶抑制活性。所有提取物均表现出抗氧化特性和对丁酰胆碱酯酶和乙酰胆碱酯酶的抑制作用；然而，乙醇提取物显示出更好的活性。