Chilling stress is a major factor limiting the yield of soya bean [Glycine max (L.) Merr.] on a global scale. However, the regulatory network that controls the chilling response of soya bean remains unclear. In the present study, phenotyping and quantitative analyses of miRNAs in soya bean under chilling stress were carried out to determine the impact of environmental constraints on soya bean productivity. Measurements done during soya bean growth in chilling along with the results of field trials indicated that the cultivars Augusta and Fiskeby V responded differently to low temperatures. Although chilling affected the reproductive development of both cultivars, the final seed output remained unchanged. The differential expression of miR169, miR319, miR397 and miR398 under cold stress was detected using ddPCR. Upon chilling in the reproductive stage, we found that these miRNAs had contrasting expression profiles in Augusta and Fiskeby V. A set of candidate target genes was predicted based on degradome sequencing data. A negative correlation was found between the expression of miR169, miR319 and miR398 and their targets in the roots of both cultivars. Our work elucidates the impact of chilling stress on the productivity of two soya bean cultivars and reveals the importance of miRNA involvement in the low temperature response.

中文翻译：

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两种大豆品种的耐冷胁迫性：表型和分子响应

低温胁迫是限制大豆产量的主要因素[ Glycine max（L.）Merr。]在全球范围内。但是，控制大豆冷响应的监管网络仍不清楚。在本研究中，进行了低温胁迫下大豆中miRNA的表型分析和定量分析，以确定环境限制条件对大豆生产力的影响。在低温条件下大豆生长过程中进行的测量以及田间试验的结果表明，奥古斯塔（Augusta）和菲斯克比（Fiskeby V）品种对低温的反应不同。虽然低温影响了两个品种的生殖发育，但最终种子产量保持不变。使用ddPCR检测冷胁迫下miR169，miR319，miR397和miR398的差异表达。在生殖阶段冷却后 我们发现这些miRNA在Augusta和Fiskeby V中具有相反的表达特征。根据降解组测序数据预测了一组候选靶基因。在两个品种的根中，miR169，miR319和miR398的表达与其靶标之间均呈负相关。我们的工作阐明了低温胁迫对两个大豆品种生产力的影响，并揭示了miRNA参与低温反应的重要性。