The monogonont rotifer Brachionus spp. have been widely used for ecotoxicological studies because of their advantages as one of the most suitable laboratory experimental species. In the present study, we obtained and assembled the whole genome sequence of the rotifer Brachionus rotundiformis, consisting of 13,612 annotated genes with 213 scaffolds and 58 Mb in total length. Focusing on ecotoxicological aspects, we conducted a comparative genome analysis on the gene families involved in detoxification, including four to six sulfotransferase gene families, seven uridine 5′-diphospho-glucuronosyltransferase gene families, and 58, 61, or 70 ATP-binding cassette genes in the genus Brachionus including Brachionus koreanus and Brachionus plicatilis. Our results suggest that these gene families have undergone a species- and/or lineage-specific evolution in response to the surrounding environmental pressure. Our genome resource for B. rotundiformis would be highly useful for future ecotoxicological studies and also provides a better understanding on the view of evolutionary mechanism of detoxification in the genus Brachionus spp.

单性轮虫Brachionus spp。由于其作为最合适的实验室实验物种之一的优势，已被广泛用于生态毒理学研究。在本研究中，我们获得并组装了轮虫Brachionus rotundiformis的全基因组序列，该序列由13,612个带注释的基因组成，具有213个支架和58 Mb的全长。针对生态毒理学方面，我们对涉及排毒的基因家族进行了比较基因组分析，包括4至6个磺基转移酶基因家族，7个尿苷5'-二磷酸-葡萄糖醛酸转移酶基因家族以及58、61或70个ATP结合盒基因在Brachionus属中，包括Brachionus koreanus和柔毛臂抱虫。我们的结果表明，这些基因家族响应周围的环境压力经历了物种和/或谱系特异性进化。我们的轮状芽孢杆菌的基因组资源将对未来的生态毒理学研究非常有用，并且可以帮助人们更好地了解Brachionus spp属的解毒进化机理。