Abstract

A measurement setup based on a transmission microscope with double acousto-optical wavelength selection is proposed for detection of high-quality spectral images with a size of 1920 × 1200 pixels in the visible wavelength range with a spectral resolution of 2.5 nm at a wavelength of 632 nm. The efficiency of the proposed approach and setup is demonstrated in the study of the transition of Misgurnus fossilis embryos from the 32nd to the 33rd stage. Correctness of the conclusions is proven in a comparison with the results of the corresponding ultrasonic study. Analysis of time dependences of the transmission spectra is performed for six spatial regions of the embryo including perivitelline space and head and tail sections. Such substantially different dependences can be used, in particular, for noninvasive monitoring and analysis of changes in the functional state of embryos related to the stage-to-stage transition.

中文翻译：

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声光视频光谱法分析生物系统中的动态过程

摘要

提出了一种基于透射显微镜和双声光波长选择的测量装置，用于检测可见光谱范围内1920×1200像素大小的高质量光谱图像，在632波长下的光谱分辨率为2.5 nm纳米 拟南芥化石过渡研究证明了所提出方法和装置的效率。胚胎从第32到第33阶段。与相应的超声研究结果相比较，证明了结论的正确性。对胚胎的六个空间区域（包括卵白质空间和头部和尾部）进行透射光谱的时间依赖性分析。这种基本上不同的依赖性可以特别地用于与阶段到阶段转换有关的胚胎的功能状态变化的无创监测和分析。