Witches’ broom disease of lime (WBDL), caused by ‘Candidatus Phytoplasma aurantifolia’, is a major limiting factor for Mexican lime (Citrus aurantifolia Swingle) production in southern Iran. Here, nutritional status and biochemical composition of healthy and infected Mexican lime in a greenhouse were compared between uninoculated (healthy) plants and plants 52 weeks after inoculation with the phytoplasma. Leaves from healthy and infected plants were sampled to measure total phenolics, antioxidant capacity, peroxidase and superoxide dismutase enzymatic activities, soluble carbohydrate, and starch. Macro- and microelements were also measured in leaves, stems, and roots of the plants. The results showed that phytoplasma infection led to a marked increase in soluble carbohydrates and starch in the leaves, but to a reduction in the roots. In leaves and roots, antioxidant capacity, phenolics, and peroxidase activity were enhanced after infection; nitrogen, calcium, boron, and manganese were significantly lower and phosphorus, magnesium, potassium, iron, zinc, and copper significantly higher in the infected leaves. This study provides new insights on metabolic and nutritional changes associated with WBDL in the greenhouse.

由“ Candidatus Phytoplasma aurantifolia”引起的女巫的石灰扫帚病（WBDL）是墨西哥石灰（Citrus aurantifolia）的主要限制因素。Swingle）在伊朗南部生产。在此，比较了未接种（健康）植物和接种植物原浆后52周的温室中健康和感染的墨西哥石灰的营养状况和生化成分。对健康和感染植物的叶子进行采样，以测量总酚，抗氧化能力，过氧化物酶和超氧化物歧化酶的酶活性，可溶性碳水化合物和淀粉。还测量了植物的叶，茎和根中的宏观和微量元素。结果表明，植物质体感染导致叶片中可溶性碳水化合物和淀粉的显着增加，但根部减少。在叶和根中，感染后抗氧化能力，酚类和过氧化物酶活性增强。氮，钙，硼，叶片中的磷和锰含量显着降低，而磷，镁，钾，铁，锌和铜的含量显着更高。这项研究提供了与温室中WBDL相关的代谢和营养变化的新见解。