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Thermotolerance and acclimation in the immature stages of Aedes aegypti (L) (Diptera: Culicidae) to simulated thermal stress
International Journal of Tropical Insect Science ( IF 1.2 ) Pub Date : 2020-07-27 , DOI: 10.1007/s42690-020-00211-x
Arun Sivan , A. N. Shriram , P. Vanamail , A. P. Sugunan

Temperature tolerance is an important factor limiting the distribution of arthropod vectors, with limited information on the ability of mosquitoes to evolve different temperature levels. Under laboratorial conditions effect of thermal stress in late third instar larvae of Aedes aegypti determined by subjecting them to temperature regimes such as 37 °C, 39 °C, 41 °C, 43 °C and 45 °C, and the lethal time required to inflict mortality of 50% (LT50) and 90% (LT90) were also computed. To understand the adaptive thermotolerance in immature stages of Ae.aegypti, late third instar larvae were exposed to 37 °C and 39 °C, and these larvae were further re-exposed to temperatures 43 °C and 45 °C respectively. The median mortality of the larvae exposed to sub-lethal temperatures at 43 °C and 45 °C was 140 and 30 min respectively. While the median mortality for the larvae adapted at 39 °C and exposed to 43 °C and 45 °C was 230 and 50 min. All the larvae exposed at 39 °C were survived up to 420 min whereas at 45 °C within 60 min of exposure time 100% mortality was observed. Larvae pre-adapted at 37 °C and 39 °C were re-exposed to 43 °C and 45 °C and it was observed that larvae pre-adapted at 39 °C were more thermotolerant than larvae pre-adapted at 37 °C. LT50 values were 82.9 and 213.7 min as compared to the non-adapted larvae. Similarly, the LT50 values were 30.5 min and in larvae pre-adapted at 39 °C when re-exposed to 45 °C showed 2 fold increases in thermotolerancein comparison to the same stage larvae kept at room temperature. Larvae pre-adapted from higher temperature showed more thermotolerance than the larvae pre-adapted at lower temperature.

中文翻译:

埃及伊蚊(L)(双翅目:虱科)未成熟阶段对模拟热应力的耐热性和驯化

温度耐受性是限制节肢动物媒介分布的重要因素,关于蚊子进化不同温度水平的能力的信息有限。在实验室条件下,热应激对埃及伊蚊晚三龄幼虫的影响是通过将它们置于 37°C、39°C、41°C、43°C 和 45°C 等温度范围内确定的,以及所需的致死时间还计算了 50% (LT50) 和 90% (LT90) 的致死率。为了了解埃及伊蚊未成熟阶段的适应性耐热性,晚三龄幼虫暴露于 37°C 和 39°C,这些幼虫分别进一步重新暴露于 43°C 和 45°C 的温度。暴露于 43°C 和 45°C 的亚致死温度下的幼虫的中位死亡率分别为 140 和 30 分钟。而幼虫适应 39°C 并暴露于 43°C 和 45°C 的中位死亡率为 230 和 50 分钟。在 39 °C 下暴露的所有幼虫都存活了 420 分钟,而在 45 °C 下暴露时间的 60 分钟内观察到 100% 死亡率。在 37°C 和 39°C 预适应的幼虫重新暴露于 43°C 和 45°C,观察到在 39°C 预适应的幼虫比在 37°C 预适应的幼虫更耐热。与未适应的幼虫相比,LT50 值为 82.9 和 213.7 分钟。类似地,LT50 值为 30.5 分钟,并且在重新暴露于 45°C 时在 39°C 下预先适应的幼虫与保持在室温下的同一阶段幼虫相比,耐热性增加了 2 倍。从较高温度预先适应的幼虫比在较低温度预先适应的幼虫表现出更高的耐热性。
更新日期:2020-07-27
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