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Maximization of micropropagule production in banana cultivars Grand naine (AAA) and Elakki (AB)
In Vitro Cellular & Developmental Biology - Plant ( IF 2.6 ) Pub Date : 2020-01-31 , DOI: 10.1007/s11627-020-10060-5
Shalini Selvakumar , Deepa Sankar Parasurama

Shoot tips from two different cultivars of banana known as Grand Naine (AAA) and Elakki (AB), were evaluated for their in vitro response in different concentrations of cytokinin and auxin which included 6-benzylaminopurine (6-BAP; 2, 3, and 4 mg L−1) and α-naphthalene acetic acid (NAA; 1 mg L−1) which was added to Murashige and Skoog (MS) basal media. The mean survival rates for cultured Grand Naine and Elakki explants in the varied growth regulator treatments were 66.25% and 52.5%, respectively. Rooting was initiated in the seventh passage using half-strength MS medium supplemented with 200 mg L−1 activated charcoal and 20 mg L−1 adenine sulfate (AS). The highest mean number of shoots generated per explant at the end of the seventh subculture from Grand Naine was 20.10 ± 0.73, and in Elakki, it was 11.20 ± 0.53. MS media supplemented with 2 mg L−1 6-BAP, and 1 mg L−1 NAA was found to be the best combination for shoot proliferation in both the cultivars. The in vitro-regenerated plants were hardened primarily under shade net conditions using cocopeat substrate and were later transferred to a 1:1:1 ratio of cocopeat, red soil, and sand mix before exposure to field conditions. The survival rate for Grand Naine and Elakki cultivars was 98.5% and 96.5%, respectively, during the acclimatization period of the micropropagules under shade net conditions. Polymorphism was not observed between the mother plant and the in vitro-regenerated micropropagules when analyzed for specific randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR).



中文翻译:

最大限度地提高香蕉品种大奈(AAA)和Elakki(AB)的微繁繁殖

在不同浓度的细胞分裂素和生长素中,包括6-苄基氨基嘌呤(6-BAP; 2,3和6 ),评估了来自两个不同品种的香蕉的尖端(Grand Naine(AAA)和Elakki(AB))的体外响应。将4 mg L -1)和α-萘乙酸(NAA; 1 mg L -1)添加到Murashige和Skoog(MS)基础培养基中。在各种生长调节剂处理下,培养的大纳奈和Elakki外植体的平均存活率分别为66.25%和52.5%。在第七代中使用补充200 mg L -1活性炭和20 mg L -1的半强度MS培养基开始生根硫酸腺嘌呤(AS)。在第七次传代结束时,来自大纳内的每个外植体产生的最高平均芽数为20.10±0.73,而在Elakki,则为11.20±0.53。两种培养基中补充有2 mg L -1 6-BAP和1 mg L -1 NAA的MS培养基是芽增殖的最佳组合。在体外-再生的植物主要在遮阳网条件下使用cocopeat基质进行硬化,然后在暴露于田间条件之前转移至比例为1:1:1的cocopeat,红壤和沙子混合料。在荫网条件下,微繁殖体适应期,Grand Naine和Elakki品种的成活率分别为98.5%和96.5%。分析特异性随机扩增多态性DNA(RAPD)和简单序列重复(SSR)时,母本植物和体外再生的微繁殖之间未观察到多态性。

更新日期:2020-01-31
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