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Therapeutic effect of hydroethanolic extract of Trianthema portulacastrum L. against N-Nitroso-N-Methylurea-induced mammary tumors in Wistar rats
Indian Journal of Traditional Knowledge ( IF 0.8 ) Pub Date : 2020-05-12
Narendra Kumar, Rajendra Singh, M Saminathan, KP Singh, K Dhama, A Arun Prince Milton, NP Kurade

This study evaluated the therapeutic action of hydroethanolic extract of Trianthema portulacastrum L. (TPE) on N-nitroso-N-methylurea (NMU)-induced mammary tumors in Wistar rats. A hydroethanolic was prepared and subjected to qualitative and quantitative phytochemical screening. After acclimatization, Wistar rats were divided into 4 groups of 6 rats each: Group A (vehicle control), Group B (TPE control), Group C (TPE treatment) and group D (NMU control). NMU (50 mg/kg body weight) was injected intraperitoneally at 50, 80 and 110 days of age. After the induction of palpable tumors, the rats were administered 200 mg/kg bw of TPE by oral gavage for 2 months. The treatment with TPE significantly (p<0.05) decreased tumor incidence, frequency, size and malignancy in comparison to the tumor-bearing rats that were not administered TPE. Immunohistochemical analysis revealed that TPE treatment significantly reduced the expression of PCNA, VEGF, ER-α and ER-β, and caused non-significant reductions in matrix metallopeptidase-9 (MMP-9). Caspase-3 expression significantly increased in TPE-treated rats in comparison with NMU-treated controls. The qRT-PCR results showed PCNA and ER-β expression was down regulated and caspase-3 expression was up regulated in the TPE-treated group. The present study showed the in vivo therapeutic action of TPE extract on NMU-induced mammary tumors. TPE exhibited antitumor activity through its antiproliferative, antiangiogenic, pro-apoptotic, and estrogen receptor-modulatory properties.

中文翻译:

木瓜的乙醇提取物对Wistar大鼠N-亚硝基-N-甲基脲诱导的乳腺肿瘤的治疗作用

这项研究评估了木瓜的乙醇提取物的治疗作用L.(TPE)对N-亚硝基-N-甲基脲(NMU)诱导的Wistar大鼠乳腺肿瘤。制备了氢乙醇,并进行了定性和定量的植物化学筛选。适应后,将Wistar大鼠分为4组,每组6只大鼠:A组(车辆对照组),B组(TPE对照组),C组(TPE处理)和D组(NMU对照组)。在50、80和110日龄时腹膜内注射NMU(50 mg / kg体重)。在诱发可触知的肿瘤后,通过口服管饲法向大鼠施用200 mg / kg bw的TPE,持续2个月。与未接受TPE的荷瘤大鼠相比,使用TPE的治疗显着(p <0.05)降低了肿瘤发生率,频率,大小和恶性程度。免疫组织化学分析显示,TPE处理可显着降低PCNA,VEGF,ER-α和ER-β,并导致基质金属肽酶9(MMP-9)的无明显减少。与NMU处理的对照组相比,在TPE处理的大鼠中Caspase-3表达显着增加。qRT-PCR结果显示,TPE治疗组的PCNA和ER-β表达下调,而caspase-3表达上调。本研究表明TPE提取物对NMU诱导的乳腺肿瘤的体内治疗作用。TPE通过其抗增殖,抗血管生成,促凋亡和雌激素受体调节特性表现出抗肿瘤活性。
更新日期:2020-05-12
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