Cisplatin (Csp) is a recurrently used chemotherapeutic drug but its use is inadequate due to undesirable adverse effects. In search of alternative medicine more attention has been given to phytochemicals. Septilin (Spt), a polyherbal drug and its therapeutic potential is huge but there is a scarcity of studies on its cytotoxic potential on cancer cells. The current study was designed to examine the effects of Spt in combination with Csp on human breast adenocarcinoma (MCF-7) and normal human breast epithelial (MCF-10A) cell lines. Cell viability for Spt treated cells was studied using MTT assay. IC₅₀ value of Csp on MCF-7 cells was found to be 10 µg/mL at 24 h. This dose was further used to study the combined effects of Csp with Spt on MCF-7 and MCF-10A cell lines. Maximum cytotoxicity of Spt on MCF-7 cells was observed at Spt 5 µg/mL. The mechanism of Spt induced cytotoxicity was studied using apoptosis assay. Spt did not show any cytotoxic effects on MCF-10 A normal human breast epithelial cells, indicating Spt has no effect on normal cells. Our findings suggest that Spt can be used in combination with an anticancer drug Csp to increase its efficacy and/or to minimize its side effects on normal cells.

中文翻译：

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Septilin对人乳腺癌细胞中顺铂细胞毒性的调节作用

顺铂（Csp）是一种经常使用的化学治疗药物，但由于不良反应而使用不足。为了寻找替代药物，人们对植物化学物质给予了更多关注。Septilin（Spt），一种多草药药物，具有巨大的治疗潜力，但对癌细胞的细胞毒性潜力研究很少。当前的研究旨在检查Spt联合Csp对人乳腺腺癌（MCF-7）和正常人乳腺上皮细胞（MCF-10A）的影响。使用MTT分析研究了Spt处理的细胞的细胞活力。IC ₅₀发现在24 h时MCF-7细胞的Csp值为10 µg / mL。该剂量进一步用于研究Csp与Spt对MCF-7和MCF-10A细胞系的联合作用。在Spt 5 µg / mL下观察到Spt对MCF-7细胞的最大细胞毒性。使用凋亡测定法研究了Spt诱导的细胞毒性的机理。Spt对MCF-10 A正常人乳腺上皮细胞未显示任何细胞毒性作用，表明Spt对正常细胞无作用。我们的发现表明，Spt可以与抗癌药物Csp结合使用，以提高其功效和/或最小化其对正常细胞的副作用。