Diabetes mellitus is a chronic disease with several comorbidities. Currently used synthetic medicines have various side effects. Herbal medicines are being widely used for management of diabetes. The present study was conducted to evaluate the effect of poly herbal formulation (PHF) (mixture of Citrullus colocynthis, Syzygium cumini and Holarrhena antidysenterica) in diabetic rats. We evaluated enzyme inhibition activity of PHF by ELISA and anti-hyperglycemic action was assessed in streptozotocin induced diabetic rats. Animals were divided into 5 groups, i.e., blank, negative control, standard control and PHF 250 and 500 mg/kg dosage respectively. Blood glucose level assessed through one touch method blood glucose monitoring system. The statistical analysis was performed by using one-way ANOVA p value of ≤0.05 was considered significant. Results of study indicated that PHF sufficiently inhibited DPPH at 0.5 mg/mL and serial dilutions with IC₅₀ of 197 µg/mL while α- glucosidase inhibition assay showed marked inhibition of PHF at 0.5 mg/mL and serial dilutions with IC₅₀ of 235 µg/mL. Oral intake of PHF reduced blood glucose level, serum triglyceride and cholesterol levels in wistar albino rats in dose dependent manner. Considering the multidirectional effects of PHF, it is concluded that PHF may be a therapeutic option in management of diabetes.

中文翻译：

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复方中草药制剂对链脲佐菌素诱导的糖尿病大鼠的降血糖和降血脂作用

糖尿病是一种慢性疾病，有多种合并症。当前使用的合成药物具有多种副作用。草药被广泛用于糖尿病的治疗。进行本研究以评估多草药配方（PHF）（西葫芦，Syzygium cumini和Holarrhena antidysenterica的混合物）的效果。在糖尿病大鼠中。我们通过ELISA评估了PHF的酶抑制活性，并评估了链脲佐菌素诱导的糖尿病大鼠的抗高血糖作用。将动物分为5组，即空白，阴性对照，标准对照和PHF 250和500 mg / kg剂量。通过一键式血糖监测系统评估血糖水平。通过使用单因素方差分析进行统计分析p值≤0.05的被认为是显著。研究结果表明，PHF足以抑制0.5 mg / mL的DPPH和IC ₅₀为197 µg / mL的系列稀释，而α-葡萄糖苷酶抑制试验显示0.5 mg / mL和IC _50的系列稀释对PHF有明显的抑制作用。235微克/毫升。口服PHF以剂量依赖性方式降低wistar白化病大鼠的血糖水平，血清甘油三酸酯和胆固醇水平。考虑到PHF的多方向作用，可以得出结论，PHF可能是治疗糖尿病的一种治疗选择。