In the present investigation, non-edible toxic oils from marine macroalgae Caulerpa racemosa species, richly available in India and composed of high calorific value and high FFA, have been selected as a feedstock for the making of biodiesel. Initially, oil extraction was carried out from the macroalgae biomass with different types of solvent systems and extraction steps. The algal oil was extracted with a solvent system consisting of 1 % diethyl ether and 10 % methylene chloride in n-hexane using ultrasonic pre-treatment technique. The algal oils were characterized by Gas Chromatography-Mass Spectrometry for composition analysis. The biodiesel was produced by transesterification method. The produced biodiesel was characterized and the conversion was calculated by Fourier-transform infrared spectroscopy (FTIR) analysis and Response Surface Methods. The fuel properties of obtained biodiesel were examined as per the American Standard Test Methods specifications in order to assess the potential of proposed biodiesel as an alternative fuel. Thus, marine macroalgae serve as a potential renewable raw-material for biodiesel production.

中文翻译：

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用花椰菜油（宏藻）油生产生物柴油

在本研究中，来自印度的海洋大型藻类Caulerpa racemosa物种的非食用有毒油已被选作生物柴油的原料，该油类在印度可得，且由高热值和高FFA组成。最初，使用不同类型的溶剂系统和萃取步骤从大型藻类生物质中进行油萃取。使用超声预处理技术，用正己烷中的1％乙醚和10％二氯甲烷组成的溶剂系统萃取藻油。藻油通过气相色谱-质谱法进行表征以进行组成分析。生物柴油是通过酯交换法生产的。对生产的生物柴油进行表征，并通过傅里叶变换红外光谱（FTIR）分析和响应面方法计算转化率。根据美国标准测试方法规范检查了获得的生物柴油的燃料特性，以评估拟议生物柴油作为替代燃料的潜力。因此，海洋大型藻类可作为生物柴油生产的潜在可再生原料。