Bioremediation, using microorganisms, especially hydrocarbon-degrading bacteria are inexpensive and eco-friendly methods to reduce oil pollutions. Plasmids containing hydrocarbon-degrading genes encode the enzymes necessary for the derivative pathways are important for bioremediation. The aim of the present work was to study the plasmid profile of bacterial strains isolated from crude-oil contaminated sites in the Persian Gulf in previous researches. In this study, plasmids were extracted from 21 strains using alkaline lysis method. Then, alkane hydroxylase gene groups (I, II, III) were detected in extracted plasmids using specific primers by PCR. The results of this study indicated that eight crude oil degrading strains out of 21 had plasmid. The results of PCR amplification confirmed that 50 % of plasmid containing strains had alkane hydroxylase gene group (I), 75 percent of them had alkane hydroxylase gene group (III) and none of them had alkane hydroxylase gene group (II).

中文翻译：

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波斯湾地区原油降解细菌中质粒谱和烷烃羟化酶基因的研究

使用微生物尤其是可降解碳氢化合物的细菌进行生物修复是减少油污染的廉价且环保的方法。含有烃降解基因的质粒编码衍生途径必需的酶对生物修复很重要。本工作的目的是研究在以前的研究中从波斯湾的原油污染场所分离出的细菌菌株的质粒图谱。在这项研究中，使用碱性裂解方法从21个菌株中提取质粒。然后，使用特异性引物通过PCR在提取的质粒中检测烷烃羟化酶基因组（I，II，III）。这项研究的结果表明，在21个原油降解菌株中，有8个具有质粒。