In the present paper, the synthesis, characterization, antimicrobial activity and in silico molecular docking study of 6-((arylidene)amino)-4-(4-chlorophenyl)-2-oxo-1-((1-(2-oxo-2H-chromen-3-yl)ethylidene)amino)-1,2-dihydropyridine-3,5-dicarbonitriles 4a-o have been reported. Compounds 4d, 4g, 4j, 4k, 4m and 4o show significant activity. Structure determination of the synthesized compounds has been done by the standard spectroscopic techniques. It is observed that biological activity is influenced by electronic environment of the molecules. Electron withdrawing group at para position plays a major role for enhancing the biological activity for antibacterial activity and the electron donating group at para position for antifungal activity. Compounds 4a-o have been further evaluated for cytotoxicity on HeLa cells. From the cytotoxicity results, compounds have been found to possess low cytotoxicity with potent antimicrobial activity.

中文翻译：

---

香豆素键合的2-吡啶酮杂环的合成，表征，分子对接研究和生物活性

本文研究了6-（（亚芳基）氨基）-4-（4-氯苯基）-2-氧代-1-（（1-（2-氧代））的合成，表征，抗菌活性和计算机分子对接研究已经报道了-2 H-铬3-（基-3-基）亚乙基）氨基）-1，2-二氢吡啶-3，5-二腈4a-o。化合物4d，4g，4j，4k，4m和4o显示出显着的活性。合成化合物的结构测定已通过标准光谱技术完成。观察到生物活性受分子的电子环境影响。电子在吸电子基团对位置起到用于增强抗菌活性和电子在供体基团的生物学活性中起主要作用的对用于抗真菌活性的位置。化合物4a-o已经进一步评估了对HeLa细胞的细胞毒性。从细胞毒性结果，发现化合物具有低的细胞毒性和有效的抗菌活性。