New tetra-azamacrocyclic complexes of Ni (II) and Cu(II) have been synthesized by template methodology leading to the formation of complex of type [MLX₂] where L is a macrcocyclic ligand obtained from the condensation of 4-Methyl-o-phenylenediamine (DAT) and 1,3-diphenylpropane-1,3-dione (DBM)and X= NO₃^-, Cl^-, and CH₃COO^-. Characterization of newly prepared complexes has been  done by using various physico-analytical techniques like UV-visible, IR, ESR, CHN, Magnetic susceptibilities and PXRD.The non-electrolytic nature of the complexes was elucidated by lower value of molar conductance. The data received from various techniques give an indication towards the octahedral geometry of the complexes. The macrocyclic ring is present at the equatorial position whereas the axial positions are occupied by the ligands Cl^-, NO₃^- and CH₃COO^-. Screening of all the complexes has been performed against the pathogenic strains of microbes in order to check their antimicrobial potential. Invitro-hemolytic activity reveals about the extent to which lyses of hemoglobin takes place. The Herring fish sperm DNA interaction studies are carried out with the help of UV-absorption spectra. Molecular modeling was done through the assistance of  software Chem 3D Ultra that gives the energy calculation and their quantum chemical parameter.

中文翻译：

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基于二价过渡金属离子的大环配合物的溶血和DNA结合研究

镍（II）和Cu（II）的新四azamacrocyclic络合物已经由模板方法合成导致的复杂类型的[MLX形成₂ ]其中，L是从缩合而得到的配位体macrcocyclic 4-甲基ø -苯二胺（DAT）和1,3-二苯基丙烷-1,3-二酮（DBM）和X = NO ₃ ^- ，氯^- ，和CH ₃ COO ^-。通过使用各种物理分析技术对新制备的配合物进行表征，例如紫外可见光，红外，ESR，CHN，磁化率和PXRD。通过较低的摩尔电导值来阐明配合物的非电解性质。从各种技术获得的数据表明了配合物的八面体几何形状。大环存在于赤道位置，而所述轴向位置由配体占据的氯^-，NO ₃ ^-和CH ₃ COO ^- 。针对微生物的致病菌株进行了所有复合物的筛选，以检查其抗菌潜力。体外-溶血活性揭示了血红蛋白裂解的程度。鲱鱼鱼精子DNA相互作用的研究是借助紫外线吸收光谱进行的。分子建模是通过Chem 3D Ultra软件进行的，该软件提供了能量计算及其量子化学参数。