Magnetic imprinted polymer having selectivity for cephalexin has been synthesized and evaluated for extraction of the antibiotic from selected food matrix. Fe₃O₄ magnetite has been prepared by co-precipitation of FeCl₂.4H₂O and FeCl₃.6H₂O in 1:2 molar ratios. Imprinted polymer has been synthesized by polymerization of methacrylic acid and ethylene glycol dimethacrylate in the presence of cephalexin over the surface of Fe₃O₄ magnetite. Selectivity of imprinted polymer over non-imprinted polymer is determined under different solvent conditions. Selectivity in both methanol and acetonitrile is found to be > 35. Selectivity is pH dependent in aqueous buffer solution and is in range of 2.33 to 21.3, maximum being at pH 5.0. Electrostatic interaction between amino group of cephalexin and carboxylic group in polymer appears to be main force contributing to selectivity of imprinted polymer. Cephalexin imprinted magnetic polymer extracted 80, 88, 63 and 78% cephalexin from water, milk, honey and egg white, respectively.

中文翻译：

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从食品基质中选择性提取头孢氨苄的磁性印迹聚合物的合成与表征

已经合成了对头孢氨苄具有选择性的磁性印迹聚合物，并对其从所选食物基质中提取抗生素进行了评估。通过以1：2的摩尔比共沉淀FeCl ₂ .4H ₂ O和FeCl ₃ .6H ₂ O来制备Fe ₃ O ₄磁铁矿。已经在Fe ₃ O ₄磁铁矿表面上存在头孢氨苄的情况下，通过甲基丙烯酸和乙二醇二甲基丙烯酸酯的聚合反应合成了印迹聚合物。在不同溶剂条件下确定印迹聚合物相对于非印迹聚合物的选择性。发现在甲醇和乙腈中的选择性均> 35。p H取决于缓冲水溶液，其范围为2.33至21.3，最大值为p H 5.0。头孢氨苄的氨基与聚合物中的羧基之间的静电相互作用似乎是影响印迹聚合物选择性的主要因素。头孢氨苄印记的磁性聚合物分别从水，牛奶，蜂蜜和蛋清中提取了80％，88％，63％和78％的头孢氨苄。