Silver nanoparticles (AgNPs) have been synthesized using Fagopyrum esculentum starch as a stabilizing and reducing agent. This reaction was carried out in an autoclave at 15 psi, 121°C for 20 min. UV-visible spectrum of the colloidal nanoparticles showed the surface plasmon absorption band with maximum absorbance at 418 nm. Interaction between functional groups present in the starch and nanoparticles were analyzed by Fourier-transform infrared spectroscopy (FTIR). Size of the synthesized nanoparticles was found to be in the range of 20-30 nm, as revealed from transmission electron microscopy (TEM). The X-ray diffraction analysis revealed the face-centred cubic (fcc) geometry of silver nanoparticles. The nanoparticles were found to be good antifungal agents against Aspergillus niger. The antibacterial activity of the nanoparticles was also studied. The nanoparticles showed higher inhibitory activity against Gram-negative bacteria (Escherichia coli) than the Gram-positive bacteria (Staphylococcus aureus). These results thus show that F. esculentum starch stabilized AgNPs could be used as a promising antimicrobial agent against bacteria the fungi In vitro cytotoxicity assessment of starch stabilized AgNPs has shown no significant cytotoxic effect on human cervical carcinoma cells lines (HeLa) by MTT assay and AgNPs concentration at 200 ug/ml of showed 86% cell viability.

中文翻译：

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荞麦淀粉绿色合成银纳米颗粒：抗真菌，抗菌活性及其细胞毒性

已使用食用荞麦淀粉作为稳定剂和还原剂合成了银纳米颗粒（AgNP）。该反应在高压釜中在15psi，121℃下进行20分钟。胶体纳米粒子的紫外-可见光谱显示表面等离激元吸收带在418 nm具有最大吸收率。淀粉和纳米颗粒中存在的官能团之间的相互作用通过傅立叶变换红外光谱（FTIR）进行了分析。如透射电子显微镜（TEM）所揭示，发现合成的纳米颗粒的尺寸在20-30nm的范围内。X射线衍射分析显示了银纳米粒子的面心立方（fcc）几何形状。发现纳米颗粒是针对黑曲霉的良好的抗真菌剂。还研究了纳米颗粒的抗菌活性。与革兰氏阳性细菌（金黄色葡萄球菌）相比，纳米颗粒对革兰氏阴性菌（大肠杆菌）具有更高的抑制活性。因此，这些结果表明，F菜淀粉稳定的AgNPs可以作为一种有前途的抗细菌真菌的抗菌剂。淀粉稳定的AgNPs的体外细胞毒性评估显示，通过MTT分析和200 ug / ml的AgNPs浓度显示86％的细胞活力。