An actinomycetes (DST-4) strain was isolated from soil of Hirakud dyke, Western Odisha, India. The isolated actinomycetes strain was subjected to 16S rRNA gene partial sequencing and the sequence in FASTA format was analysed at National Centre Biotechnology Information (NCBI) portal using basic local alignment search tool (BLAST) analysis. The sequence was identified as a strain of gentamicin producing Micromonosperma echinospora with 99% identify. The sequence was submitted to GenBank and obtained an Accession no. MF536417.1. A phylogenetic tree was generated with six strains of same species available in database. The actinomycetes strain (DST-4) was screened for its antagonistic activity against three streptomycin resistant Staphylococcal aureus strains. ‘Triplicate patch inoculums diffusion’ and ‘cell immersed tube dilution’ techniques were used. Streptomycin was taken as reference drug. A quantitative cocultured-incubation method was applied for induction of bioactive compounds from the isolated actinomycete strain. The growth curve analysis of test staphylococcal strains through spectrophotometric as well as subculture experiments had inferred about antibacterial activity of putative bioactive compounds present in cell free extract (CFE) of DST-4 strain. The H¹ NMR study of high performance liquid chromatography (HPLC) chromatogram peak fraction of CFE, was carried out. The nuclear magnetic resonance (NMR) signals had inferred about induction bioactive compounds with of R₂NH and ROH as functional groups.

中文翻译：

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从隐蔽的Hirakud堤防土壤中分离的微型单眼棘孢菌（DST-4）共培养菌株中的生物活性化合物的诱导

从印度西部奥里萨邦的Hirakud堤防土壤中分离了放线菌（DST-4）菌株。对分离的放线菌菌株进行16S rRNA基因部分测序，并使用基本的局部比对搜索工具（BLAST）在国家生物技术信息中心（NCBI）门户网站上分析FASTA格式的序列。该序列被鉴定为庆大霉素的产生菌株Micromonosperma棘用99％鉴定。该序列被提交给GenBank并获得了登录号。MF536417.1。用数据库中可用的六个相同物种的菌株产生了系统发育树。筛选放线菌菌株（DST-4）对三种链霉素抗性金黄色葡萄球菌的拮抗活性株。使用了“三重斑块接种扩散”和“细胞浸没管稀释”技术。链霉素被用作参考药物。定量共培养孵育方法应用于从分离的放线菌菌株中诱导生物活性化合物。通过分光光度法和继代培养实验对测试葡萄球菌菌株的生长曲线分析推断出存在于DST-4菌株无细胞提取物（CFE）中的假定生物活性化合物的抗菌活性。进行了CFE的高效液相色谱（HPLC）色谱峰分数的H ¹ NMR研究。核磁共振（NMR）信号已推断出具有R ₂ NH和ROH作为官能团的诱导生物活性化合物。