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Molecular analysis of fenazaquin selected resistant strain of two-spotted spider mite Tetranychus urticae Koch
Indian Journal of Biochemistry and Biophysics ( IF 1.476 ) Pub Date : 2018-11-12
Rakesh Kumar Sharma, Manmeet Brar Bhullar, Satnam Singh, Vikas Jindal

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) is an important agricultural pest in a wide range of outdoor and protected crops worldwide. Fenazaquin is mitochondrial electron transport inhibition (METI)-acaricide and its extensive and frequent use for control of this mite has facilitated resistance development. So, present studies were conducted to investigate genetic differences between fenazaquin resistant and susceptible population of T. urticae as no information is available regarding mutation/variability in genes involved in imparting resistance. Fenazaquin selected resistant population was developed in the laboratory by giving selection pressure for up to 15 generations which resulted into 166.49 fold increase in resistance level when compared with susceptible population. Molecular analysis of resistant and susceptible population revealed no changes in genes structure of CYP392A11 and CYP392A12 in the resistant compared to the susceptible population. However, expression profiling of nine different genes associated (CYP392A11, CYP392A12, CYP392A16, CYP392D2, CYP392D3, CYP392D6, CYP392D7, CYP392D8 and CYP392D10p) with METI resistance showed increased mRNA transcripts in CYP392A11, CYP392A12, CYP392D2 and CYP392D10p. The increased MFO activity in the resistant population corresponds to nearly two fold increase in the expression of CYP392A11 and CYP392A12. The target site mutation might not have any role in low to moderate level of fenazaquin resistance in mites. Differences in mRNA expression are a simple, fast and reliable tool for early detection of resistance.

中文翻译:

Fenazaquin选择的两斑叶螨Tetranychus urticae Koch抗药性菌株的分子分析

二斑叶螨Tetranychus urticae Koch(Acari:Tetranychidae)是一种重要的农业害虫,在全球范围内广泛存在于户外和受保护的农作物中。Fenazaquin是线粒体电子运输抑制(METI)杀螨剂,其广泛且频繁地用于控制该螨虫已促进了抗药性的发展。因此,目前的研究进行了调查,以调查耐氟苯喹喹和易感性锥虫种群之间的遗传差异因为尚无有关抗药性基因突变/变异性的信息。Fenazaquin筛选出的抗性种群是在实验室中通过给予多达15代的选择压力而开发的,与易感种群相比,该抗性水平提高了166.49倍。对耐药人群和易感人群的分子分析表明,与易感人群相比,CYP392A11CYP392A12的基因结构没有变化。然而,与METI抗性相关的9种不同基因(CYP392A11,CYP392A12,CYP392A16,CYP392D2,CYP392D3,CYP392D6,CYP392D7,CYP392D8CYP392D10p)的表达显示mRNA转录增加CYP392A11,CYP392A12,CYP392D2CYP392D10p。在抗性人群中增加的MFO活性对应于CYP392A11和CYP392A12的表达增加近两倍。靶位点突变可能对螨的低至中等水平的耐Fenazaquin没有任何作用。mRNA表达的差异是早期检测耐药性的简单,快速和可靠的工具。
更新日期:2018-11-12
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