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Isolation and amplification of genomic DNA from nutmeg mace
Indian Journal of Biochemistry and Biophysics ( IF 1.476 ) Pub Date : 2018-10-23
V P Swetha, V A Parvathy, T E Sheeja, B Sasikumar

A reliable and efficient protocol for isolation and amplification of genomic DNA from dried mace of Myristica fragrans, was developed. The yield of genomic DNA was 231.4 µg g-1 and 306.8 µg g-1, respectively for the samples procured from the farm and market. The absorbance ratio at A260/A280 was greater than 1.8 indicating the good quality of DNA. Complete restriction digestion and PCR amplification of genomic DNA further confirmed the quality of isolated DNA.

中文翻译:

肉豆蔻钉的基因组DNA的分离和扩增

开发了一种可靠和有效的方案,用于从干燥的肉豆蔻中提取和扩增基因组DNA 。从农场和市场采购的样品的基因组DNA产量分别为231.4 µg g -1和306.8 µg g -1。在A260 / A280处的吸光度比大于1.8,表明DNA质量良好。基因组DNA的完全限制性消化和PCR扩增进一步证实了分离DNA的质量。
更新日期:2018-10-23
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