Salinity and drought are the two major abiotic stresses that affect the quality and quantity of tomato. The dehydration response transcription element binding factor (DREB) gene enhances tolerance in plants against salinity. The present study was conducted to transformed DREB1A gene through Agrobacterium in tomato cultivar Roma against salt stresses. A binary vector containing DREB1A gene under the control of inducible promoter Lip 9 along with hygromycin screening marker was used. The few major factors which directly affect transformation efficiency were optimized such as acetosyringone levels, cefotaxime concentrations, pre-selection time period and hygromycin levels. Four different concentrations (50, 100, 150 and 200 mM) of NaCl were applied on both transgenic and non transgenic plants. The addition of 200 µM acetosyringone in co-cultivation media, 200 mg/L cefotaxime in pre-culturing media and 25 mg/L hygromycin concentration significantly increased transformation efficiency. Maximum transformation efficiency 16.6% was obtained in tomato cultivar Roma. The transgenic plants were confirmed with PCR and the expected band size ~630 bp was amplified. The transgene express in transgenic tomatoes was confirmed by reverse transcriptase- polymerase chain reaction (RT-PCR). The resulted transgenic plants showed enhanced tolerance against high salt concentration (200 mM) while the non transgenic plants showed the symptoms of wilting and eventually die at that concentration. The DREB1A gene was successfully transformed into local tomato cultivar Roma. The resulted transgenic tomato cultivar Roma will be recommended against extreme salt stresses.

中文翻译：

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番茄（Solanum lycopersicum）抗盐胁迫的遗传改良。

盐度和干旱是影响番茄质量和数量的两个主要非生物胁迫。脱水反应转录元件结合因子（DREB）基因增强了植物对盐分的耐受性。本研究旨在通过农杆菌转化番茄品种罗马中的DREB1A基因抗盐胁迫。包含DREB1A的二进制向量使用在诱导型启动子Lip 9控制下的Hyperi基因和潮霉素筛选标记。优化了直接影响转化效率的几个主要因素，例如乙酰丁香酮水平，头孢噻肟浓度，预选时间段和潮霉素水平。将四种不同浓度（50、100、150和200 mM）的NaCl应用于转基因和非转基因植物。在共培养培养基中添加200 µM乙酰丁香酮，在预培养培养基中添加200 mg / L头孢噻肟和25 mg / L潮霉素浓度可显着提高转化效率。在番茄品种罗马中获得最高转化效率16.6％。通过PCR确认了转基因植物，并扩增了预期的〜630 bp的条带大小。通过逆转录酶-聚合酶链反应（RT-PCR）证实了转基因番茄中的转基因表达。所得的转基因植物显示出对高盐浓度（200 mM）的增强的耐受性，而非转基因植物显示出枯萎的症状，并最终在该浓度下死亡。的DREB1A基因成功地转化为本地番茄品种罗马。推荐使用转基因番茄栽培品种罗马来抵抗极端的盐胁迫。