Azadirachta indica, popularly known as ‘Neem’, is an important plant in the Ayurveda system of medicine. It is known to prevent about at least 40 types of diseases in home-practice. A previous report showed that the methanolic extract of this plant can effectively control proliferation of leukemia cells. Here, we explored the eEthanolic extract dried neem leaves for a new molecule with potent anti-leukemic property.Four biomolecules were isolated viz. rutin, isoquercetin, quercetrin and β-sitosterol-3-O-β-D-glucoside from the ethanolic extract by repeated column chromatography and HPLC.Quercetrin structures of the isolated molecules were confirmed by Mass, ¹HNMR, ¹³C NMR spectra analysis. MTT assay revealed that β-sitosterol-3-O-β-D-glucoside effectively reduced the proliferation of MOLT 4 leukemic cells in a dose and time dependent manner. DAPI staining with confocal microscopy indicated that β-sitosterol-3-O-β-D-glucoside efficiently induced nuclear DNA fragmentation in MOLT 4 cells. Finally, flow cytometry after PI staining showed that the compound has potential to check the cell cycle progression at sub G1 phase. With the available results, it can be concluded that β-sitosterol-3-O-β-D-glucoside has potential to be good therapeutic drug in leukemia treatment.

中文翻译：

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从印A中分离得到的新型化合物β-谷甾醇-3-O-β-D-葡萄糖苷通过靶向G0 / G1人群有效诱导白血病细胞凋亡

印楝，俗称为“楝树”，是医学的阿育吠陀体系中的重要设备。众所周知，在家中至少可以预防40种疾病。先前的报道显示该植物的甲醇提取物可以有效地控制白血病细胞的增殖。在这里，我们探讨了eEthanolic提取干楝树叶与强效的抗白血病property.Four生物分子的新分子分离即。乙醇提取物中的芦丁，异槲皮素，槲皮素和β-谷甾醇-3-O-β-D-葡萄糖苷通过重复柱色谱和HPLC进行分离。质谱，¹ HNMR，¹³证实了分离的分子的槲皮素结构1 H NMR光谱分析。MTT分析显示β-谷甾醇-3-O-β-D-葡萄糖苷以剂量和时间依赖性方式有效地降低了MOLT 4白血病细胞的增殖。共聚焦显微镜DAPI染色表明，β-谷甾醇-3-O-β-D-葡萄糖苷可有效诱导MOLT 4细胞中的核DNA片段化。最后，PI染色后的流式细胞术表明该化合物具有检查亚G1期细胞周期进程的潜力。根据可获得的结果，可以得出结论，β-谷甾醇-3-O-β-D-葡萄糖苷有潜力成为白血病治疗中的良好治疗药物。