Diabetes mellitus has become a global epidemic as its prevalence is steadily increasing everywhere at an alarming rate, most markedly in the India. Traditional Indian system of medicine based on herbal therapies is being practiced since ancient times and has been proved very effective against number of diseases. Trigonella foenum-graecum (Tfg) and Cinnamomum zeylanicum (Cz), the two spices, possess multiple medicinal properties. Hence present study is aimed to evaluate the protective effects of these plants on the oxidative DNA damage in diabetic rats. The presence of DNA base oxidation was investigated by modified comet assay which uses bacterial repair enzymes, formamidopyrimidine glycosylase and Endonuclease III which can measure oxidatively damaged purines and pyrimidines, respectively. Diabetes induced alteration in total antioxidant capacity (TAC), generation of superoxide anion and the levels of GSH, GSSG, NADH, NAD⁺, NADPH and NADP⁺ were monitored. Diabetes was induced by intravenous injection of alloxan, followed by oral administration of aqueous suspension of Tfg seeds and bark of Cz. Treatment of Tfg and Cz significantly restored the altered level of superoxide anion, TAC, ratios of GSH/GSSG, NAD(P)H/NAD(P)⁺ and NADH/NAD⁺ in the diabetic group. Furthermore, both the treatment effectively decreased the level of oxidatively damaged purine and pyrimidine bases.

中文翻译：

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胡芦巴和肉桂对大鼠糖尿病引起的氧化性DNA损伤的保护作用

糖尿病已成为全球流行病，因为它的流行率以惊人的速度稳定增长，在印度尤为明显。自古以来就开始实行基于草药疗法的传统印度医学体系，并且已被证明对许多疾病非常有效。胡芦巴（Tfg）和肉桂（Cznamanium zeylanicum（Cz），这两种香料具有多种药用特性。因此，本研究旨在评估这些植物对糖尿病大鼠氧化DNA损伤的保护作用。通过使用细菌修复酶，甲酰胺基嘧啶糖基化酶和核酸内切酶III的改良彗星试验研究了DNA碱基氧化的存在，该酶可以分别测量氧化损伤的嘌呤和嘧啶。监测糖尿病引起的总抗氧化能力（TAC），超氧阴离子的生成以及GSH，GSSG，NADH，NAD ⁺，NADPH和NADP ⁺的变化。静脉内注射四氧嘧啶诱导糖尿病，然后口服Tfg种子水悬浮液和Cz树皮。Tfg和Cz的治疗可显着恢复糖尿病组中超氧阴离子，TAC，GSH / GSSG，NAD（P）H / NAD（P）⁺和NADH / NAD ⁺的改变水平。此外，两种处理均有效降低了氧化损伤的嘌呤和嘧啶碱基的水平。