Endometriosis (EM) is an estrogen-dependent, frequent benign gynecological disease, which reduces the quality of life in EM patients. Propofol regulates the apoptosis of EM. miR-9-5p is decreased in early secretory endometrium, TGFBI is increased in EM, however, their functions in EM remain unclear. This study aimed to investigate the mechanisms underlying the effects of propofol on the proliferation and invasion of EM by regulating miR-9-5p/TGFBI. The proliferation and invasion of ectopic endometrial stromal cells (ESCs) were determined by MTT assay and Transwell assay, respectively. Real-time PCR was applied for the evaluation of miR-9-5p and transforming growth factor-β (TGF-β)-induced gene (TGFBI) mRNA level in ESCs. Western blot was used to examine the protein level of TGFBI protein (TGFBIp) in ESCs. The relation between miR-9-5p and TGFBI was predicted by TargetScan 7.1 and verified by dual luciferase reporter assay. Propofol reduced cell proliferation and invasion, while induced miR-9-5p level and reduced TGFBI/TGFBIp level in ectopic ESCs. Additionally, in ectopic ESCs from patients with EM, miR-9-5p was lower, while TGFBI and TGFBIp were higher than those from eutopic endometrium and normal endometrium from patients with hysteromyoma. Moreover, propofol reduced proliferation and invasion in ectopic ESCs, which was realized by the regulation of miR-9-5p/TGFBI.

子宫内膜异位症（EM）是雌激素依赖性的常见良性妇科疾病，会降低EM患者的生活质量。异丙酚调节EM的凋亡。早期分泌性子宫内膜中miR-9-5p降低，EM中TGFBI升高，但是，它们在EM中的功能仍不清楚。本研究旨在通过调节miR-9-5p / TGFBI来研究异丙酚对EM增殖和侵袭的潜在机制。通过MTT法和Transwell法分别测定异位子宫内膜间质细胞（ESCs）的增殖和侵袭能力。实时荧光定量PCR用于评估ESC中miR-9-5p和转化生长因子-β（TGF-β）诱导基因（TGFBI）的mRNA水平。用蛋白质印迹法检查ESC中TGFBI蛋白（TGFBIp）的蛋白水平。通过TargetScan 7.1预测了miR-9-5p与TGFBI之间的关系，并通过双重萤光素酶报告基因检测证实了这一点。异丙酚可降低细胞增殖和侵袭，同时在异位ESC中诱导miR-9-5p水平并降低TGFBI / TGFBIp水平。另外，在EM患者的异位ESC中，miR-9-5p较低，而TGFBI和TGFBIp高于子宫肌瘤患者的异位子宫内膜和正常子宫内膜。而且，丙泊酚减少了异位ESC中的增殖和侵袭，这是通过调节miR-9-5p / TGFBI实现的。子宫肌瘤患者的TGFBI和TGFBIp高于异位子宫内膜和正常子宫内膜。而且，丙泊酚减少了异位ESC中的增殖和侵袭，这是通过调节miR-9-5p / TGFBI实现的。而子宫肌瘤患者的TGFBI和TGFBIp高于异位子宫内膜和正常子宫内膜。此外，丙泊酚减少了异位胚胎干细胞的增殖和侵袭，这是通过调节miR-9-5p / TGFBI实现的。