The aim of this study was to investigate the rat small intestine mesentery and colon as natural bio‐reactors for rat colon‐derived scaffolds. We decellularized eight whole rat colons by a perfusion‐based protocol using 0.1% sodium dodecyl sulfate for 24 hr. The provided bio‐scaffolds were examined by histological staining, scanning electron microscopy, and collagen and sulfated glycosaminoglycan quantification. Subsequently, we implanted 4 cm segments of the provided bio‐scaffolds into two groups of animal models comprising tissue grafting into the mesenteric tissue (n: 10) and end‐to‐end anastomosis (n: 10) to the colon of host rats. Following 9 months of follow‐up, we harvested the grafts and performed histological and immunohistochemical studies as well as real‐time PCR evaluation for telomerase activity of the samples. Histological staining, scanning electron microscopy and protein content evaluation of the acellular tissues confirmed the complete removal of the cellular components and preservation of the extracellular matrix. Histopathological assessment of the implanted scaffolds was suggestive of a regenerative process in both groups. Moreover, immunohistochemical analysis of the samples confirmed the presence of smooth muscle cells, endothelial progenitor cells, and neural elements in both groups of grafted scaffolds. Our data confirmed the recellularization of the acellular colon grafts in both groups after 9 months of follow up. Also, the implanted tissues demonstrated different characteristics based on their implantation location. The outcomes of this investigation illustrate the capability of acellular tissues for in vivo application and regeneration.

中文翻译：

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随访9个月后，体内应用了脱细胞的大鼠结肠并评估了工程支架。

本研究的目的是研究大鼠小肠肠系膜和结肠作为大鼠结肠衍生支架的天然生物反应器。我们使用0.1％十二烷基硫酸钠通过基于灌注的方案将8个完整的大鼠结肠脱细胞24小时。通过组织学染色，扫描电子显微镜，胶原蛋白和硫酸化的糖胺聚糖定量检查所提供的生物支架。随后，我们将所提供的生物支架的4 cm片段植入两组动物模型中，包括将组织移植到肠系膜组织（n：10）和端到端吻合（n：10）到宿主大鼠的结肠。经过9个月的随访，我们收获了移植物，并进行了组织学和免疫组化研究，以及实时PCR评估样品的端粒酶活性。对无细胞组织的组织学染色，扫描电子显微镜和蛋白质含量评估证实了细胞成分的完全去除和细胞外基质的保存。植入支架的组织病理学评估提示两组均具有再生过程。此外，样品的免疫组织化学分析证实两组移植支架中均存在平滑肌细胞，内皮祖细胞和神经元。我们的数据证实了随访9个月后两组无细胞结肠移植物的再细胞化。也，植入的组织根据其植入位置表现出不同的特征。这项研究的结果说明了脱细胞组织在体内应用和再生的能力。