This study demonstrates both the antioxidant and anticancer potential of the novel short molecule YT12 derived from peroxiredoxin (Prx) of spirulina, Arthrospira platensis (Ap). ApPrx showed significant reduction in reactive oxygen species (ROS) against hydrogen peroxide (H₂O₂) stress. The complementary DNA sequence of ApPrx contained 706 nucleotides and its coding region possessed 546 nucleotides between position 115 and 660. Real‐time quantitative reverse transcription polymerase chain reaction analysis confirmed the messenger RNA expression of ApPrx due to H₂O₂ exposure in spirulina cells at regular intervals, in which the highest expression was noticed on Day 20. Cytotoxicity assay was performed using human peripheral blood mononuclear cells, and revealed that at 10 μM, the YT12 did not exhibit any notable toxicity. Furthermore, ROS scavenging activity of YT12 was performed using DCF‐DA assay, in which YT12 scavenged a significant amount of ROS at 25 μM in H₂O₂‐treated blood leukocytes. The intracellular ROS in human colon adenocarcinoma cells (HT‐29) was regulated by oxidative stress, where the YT12 scavenges ROS in HT‐29 cells at 12.5 μM. Findings show that YT12 peptide has anticancer activity, when treated against HT‐29 cells. Through the MTT assay, YT12 showed vital cytotoxicity against HT‐29 cells. These finding suggested that YT12 is a potent antioxidant molecule which defends ROS against oxidative stress and plays a role in redox balance.

中文翻译：

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拟南芥来源的短分子YT12的Peroxiredoxin影响抗氧化和抗癌活性。

这项研究证明了源自螺旋藻，拟南芥（Aphrospira platensis）（Ap）的过氧iredoxin（Prx）的新型短分子YT12的抗氧化和抗癌潜力。Ap Prx对过氧化氢（H ₂ O ₂）应力显示出活性氧（ROS）的显着降低。Ap Prx的互补DNA序列包含706个核苷酸，其编码区在115位和660位之间具有546个核苷酸。实时定量逆转录聚合酶链反应分析证实了H ₂ O ₂导致Ap Prx的信使RNA表达。定期暴露于螺旋藻细胞中，在第20天观察到最高表达。使用人外周血单核细胞进行了细胞毒性测定，结果表明，在10μM的浓度下，YT12没有表现出任何明显的毒性。此外，使用DCF‐DA分析法对YT12的ROS清除活性进行了测定，其中YT12在H ₂ O _2中以25μM的量清除了大量ROS。经治疗的血液白细胞。人结肠腺癌细胞（HT-29）的细胞内ROS受氧化应激的调节，其中YT12以12.5μM清除HT-29细胞中的ROS。研究结果表明，当针对HT-29细胞治疗时，YT12肽具有抗癌活性。通过MTT分析，YT12对HT-29细胞显示出至关重要的细胞毒性。这些发现表明YT12是一种有效的抗氧化剂分子，可以保护ROS抵御氧化应激，并在氧化还原平衡中发挥作用。