The transition from 2D to 3D engineered tissue cultures is changing the way biologists can perform in vitro functional studies. However, there has been a paucity in the establishment of methods required for the generation of microdevices and cost‐effective scaling up. To date, approaches including multistep photolithography, milling and 3D printing have been used that involve specialized and expensive equipment or time‐consuming steps with variable success. Here, a fabrication pipeline is presented based on affordable off‐the‐shelf 3D printers and novel replica molding strategies for rapid and easy in‐house production of hundreds of 3D culture devices per day, with customizable size and geometry. This pipeline is applied to generate tissue engineered skeletal muscles in vitro using human induced pluripotent stem cell‐derived myogenic progenitors. These production methods can be employed in any standard biomedical laboratory.

中文翻译：

---

用于骨骼肌组织工程设备内部制造的3D打印和新型复制品成型的耦合

从2D到3D工程组织培养的转变正在改变生物学家进行体外功能研究的方式。但是，建立微型设备和经济有效地扩大规模所需的方法的建立很少。迄今为止，已经使用了包括多步光刻，铣削和3D打印在内的方法，这些方法涉及专用且昂贵的设备或耗时的步骤，但取得了成功。在这里，提出了一种基于价格合理的现成3D打印机和新颖的复制成型策略的制造流程，该策略可以快速，简便地内部每天生产数百个3D培养设备，并具有可自定义的尺寸和几何形状。该管道可用于使用人类诱导的多能干细胞衍生的成肌祖细胞在体外生成组织工程化的骨骼肌。