Pectinases are enzymes which are widely distributed in microbes that are present in pectin enriched sites. The agro-industrial residues can be utilized in the industrial scale for low-cost and efficient pectinase production in an eco-friendly approach. This study employs low-cost substrates (i.e. culinary fruit peels) for maximum pectinase production from novel Streptomyces fumigatiscleroticus VIT-SP4. The extraction and characterization of pectin from different fruit peels were investigated and pectinase activity was analyzed. The orange pectin gave maximum pectinase activity of about 45.93 (U/mL). Further, statistical optimization of process parameters was studied by using Taguchi method showed optimum values of pH-6, temperature −35 °C, orange pectin% − 2.5, incubation time- 48 h and RPM- 200 rpm and pectinase activity was found to be 98.65 (U/mL). The response surface methodology (RSM) was used for the optimization of media components which revealed that starch −1.17%, yeast extract-2%, and orange pectin% − 0.75% produces maximum pectinase of about 170.05 (U/mL). The drug-delivery study showed drug release was not observed at initial pH 3 after 4 h. The immediate drug release was noted at pH 6 caused due to disintegration of pectin by the pectinase activity. The self-healing of cracks by spray culture technique was investigated. The crack healing was observed up to 0.50 mm wide after 12 days. This confirms the ability of actinomycete spores to survive and they react to form calcite complex directly helps in crack healing process. This low-cost microbial pectinase can be used in drug delivery and concrete crack-healing applications sectors in future.

果胶酶是广泛分布在存在于富含果胶的位点的微生物中的酶。农工业残留物可用于工业规模，以生态友好的方式低成本高效地生产果胶酶。本研究采用低成本底物（即烹饪水果皮）从新型Streptomyces fumigatiscleroticus中最大限度地生产果胶酶维生素-SP4。研究了不同果皮中果胶的提取和表征，并分析了果胶酶活性。橙色果胶的最大果胶酶活性约为 45.93 (U/mL)。此外，通过使用田口方法研究了工艺参数的统计优化，表明 pH-6、温度-35°C、橙果胶%-2.5、孵育时间-48 小时和 RPM-200 rpm 的最佳值，发现果胶酶活性为98.65 (U/mL)。响应面法 (RSM) 用于优化培养基成分，结果表明淀粉 -1.17%、酵母提取物-2% 和橙果胶% - 0.75% 产生的果胶酶最大值约为 170.05 (U/mL)。药物递送研究表明，在初始 pH 值为 3 时 4 小时后未观察到药物释放。由于果胶酶活性对果胶的分解，在 pH 6 时注意到药物立即释放。研究了喷雾培养技术对裂缝的自我修复。12 天后观察到裂纹愈合达 0.50 毫米宽。这证实了放线菌孢子的生存能力，它们反应形成方解石复合物直接有助于裂缝愈合过程。这种低成本的微生物果胶酶未来可用于药物输送和混凝土裂缝修复应用领域。