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Regenerative potential of pluripotent nontumorgenetic stem cells: Multilineage differentiating stress enduring cells (Muse cells)
Regenerative Therapy ( IF 4.3 ) Pub Date : 2020-07-27 , DOI: 10.1016/j.reth.2020.04.011
Jiankun Cao , Zhigang Yang , Ran Xiao , Bo Pan

Multilineage differentiating stress enduring cells (Muse cells), double positive for SSEA-3 and CD105, can be isolated by fluorescence-activated cell sorting (FACS) or sever cellular conditions from dermal fibroblasts, bone marrow stem cells (BMSCs), adipose tissue derived stem cells (ADSCs), fresh bone marrow and liposuction fat. When cultured in a single-cell suspension, Muse cells can grow into characteristic cell clusters. Muse cells maintain pluripotency as evidenced by pluripotent markers in vitro. Besides, Muse cells have no tumorigenesis up to 6 months in SCID mice. Muse cells differentiate into cells representative of all three germ layers both spontaneously and under specific induction. In comparison to mesenchymal stem cells (MSCs), Muse cells show higher homing and migration capabilities to damaged sites which is predominantly attributed to S1P–S1PR2 axis. The regenerative effects of Muse cells have been demonstrated by many models in vivo or in vitro, including stroke, intracerebral hemorrhage, myocardial infarction, aortic aneurysm, lung injuries, liver fibrosis, focal segmental glomerulosclerosis, osteochondral defects and skin ulcer. In general, migration, differentiation and paracrine play a pivotal role in the regeneration capability. Here we review the isolation, core properties, preclinical studies as well as the underling molecular and cellular details to highlight their regenerative potential.



中文翻译:

多能非肿瘤干细胞的再生潜能:多系分化应激持久细胞(Muse细胞)

可以通过荧光激活细胞分选(FACS)或重度细胞条件从真皮成纤维细胞,骨髓干细胞(BMSCs),脂肪组织中分离出SSEA-3和CD105呈双阳性的多系分化应激持久细胞(Muse细胞)干细胞(ADSC),新鲜的骨髓和吸脂脂肪。当在单细胞悬液中培养时,Muse细胞可以生长成特征性的细胞簇。缪斯细胞可保持多能性,体外多能性标志物证明了这一点。此外,SCID小鼠长达6个月的Muse细胞无肿瘤发生。缪斯细胞自发地和在特异性诱导下分化成代表所有三个胚层的细胞。与间充质干细胞(MSC)相比,缪斯细胞表现出更高的归巢和迁移能力,主要归因于S1P–S1PR2轴。Muse细胞的再生作用已在许多体内或体外模型中得到证实,包括中风,脑出血,心肌梗死,主动脉瘤,肺损伤,肝纤维化,局灶性节段性肾小球硬化,骨软骨缺损和皮肤溃疡。通常,迁移,分化和旁分泌在再生能力中起关键作用。在这里,我们回顾了分离,核心特性,临床前研究以及基础的分子和细胞细节,以突出它们的再生潜力。心肌梗塞,主动脉瘤,肺损伤,肝纤维化,局灶性节段性肾小球硬化,骨软骨缺损和皮肤溃疡。通常,迁移,分化和旁分泌在再生能力中起关键作用。在这里,我们回顾了分离,核心特性,临床前研究以及基础的分子和细胞细节,以突出它们的再生潜力。心肌梗塞,主动脉瘤,肺损伤,肝纤维化,局灶性节段性肾小球硬化,骨软骨缺损和皮肤溃疡。通常,迁移,分化和旁分泌在再生能力中起关键作用。在这里,我们回顾了分离,核心特性,临床前研究以及基础的分子和细胞细节,以突出它们的再生潜力。

更新日期:2020-07-27
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