The present study was designed to evaluate the possible effects of the paediatric vaccination schedule in the United States on the central nervous system in a murine model. We compared the impact of treatment with the whole vaccines versus true placebo control. Seventy-six pups were divided into three groups: two vaccinated groups and unvaccinated control. The two vaccinated groups were treated between 7 and 21 post-natal days either with one or three times of the vaccine doses per body weight as used in children between newborn and eighteen months of age. The post-vaccination development, neuromotor behaviours and neurobehavioural abnormalities (NBAs) were evaluated in all mouse groups during the 67 post-natal weeks of mouse age. Mouse body weight was affected only in the vaccinated females compared to males and control. Some NBAs such as decreased sociability, increased anxiety-like behaviours, and alteration of visual-spatial learning and memory were observed in vaccinated male and female mice compared to controls. The present study also shows a slower acquisition of some neonatal reflexes in vaccinated female mice compared to vaccinated males and controls. The observed neurodevelopmental alterations did not show a linear relationship with vaccine dose, suggesting that the single dose gave a saturated response. The outcomes seemed to be sex-dependent and transient with age.

本研究旨在评估美国儿科疫苗接种计划对小鼠模型中枢神经系统的可能影响。我们比较了全疫苗治疗与真正安慰剂对照治疗的影响。76 只幼崽被分成三组：两个接种疫苗的组和未接种疫苗的对照组。两个接种组在出生后 7 至 21 天之间接受治疗，疫苗剂量是新生儿至 18 个月大的儿童所用疫苗剂量的 1 或 3 倍。在小鼠出生后 67 周内评估所有小鼠组的接种后发育、神经运动行为和神经行为异常 (NBA)。与雄性和对照相比，小鼠体重仅在接种的雌性中受到影响。与对照组相比，在接种疫苗的雄性和雌性小鼠中观察到了一些 NBA，例如社交能力下降、焦虑样行为增加以及视觉空间学习和记忆的改变。本研究还表明，与接种疫苗的雄性和对照组相比，接种疫苗的雌性小鼠获得一些新生儿反射的速度较慢。观察到的神经发育改变与疫苗剂量没有线性关系，表明单剂量产生了饱和反应。结果似乎与性别有关，并且随着年龄的增长而短暂。本研究还表明，与接种疫苗的雄性和对照相比，接种疫苗的雌性小鼠获得一些新生儿反射的速度较慢。观察到的神经发育改变与疫苗剂量没有线性关系，表明单剂量产生了饱和反应。结果似乎与性别有关，并且随着年龄的增长而短暂。本研究还表明，与接种疫苗的雄性和对照相比，接种疫苗的雌性小鼠获得一些新生儿反射的速度较慢。观察到的神经发育改变与疫苗剂量没有线性关系，表明单剂量产生了饱和反应。结果似乎与性别有关，并且随着年龄的增长而短暂。