当前位置: X-MOL 学术Dev. Comp. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Fish lysozyme gene family evolution and divergent function in early development.
Developmental & Comparative Immunology ( IF 2.9 ) Pub Date : 2020-07-27 , DOI: 10.1016/j.dci.2020.103772
Lisen Li 1 , João C R Cardoso 1 , Rute C Félix 1 , Ana Patrícia Mateus 1 , Adelino V M Canário 1 , Deborah M Power 1
Affiliation  

Lysozymes are an ancient group of antimicrobial enzymes of the innate immune system. Here we provide a comparative analysis of the evolution and function of lysozymes during early development in fish, the most speciose vertebrate group. In fishes, lineage and species-specific evolution of both C-type (chicken or conventional) and G-type (goose type) genes occurred. Phylogenetic analysis revealed that the teleost lysozyme G-type members group with the tetrapod homologues but the teleost C-type form three different clusters with the tetrapods. Most of the teleost C-type cluster with tetrapod Lyz but there are some that group with the mammalian Lyzl1/2 and LALBA. This suggests that early in gnathostome evolution these genes already existed and that lyzl1/2 and lalba genes are present in fish and tetrapods. Gene synteny analysis to confirm sequence orthologies failed to identify conserved genome regions between teleosts and other vertebrates lysozyme gene regions suggesting that in the ancestral bony fish genome lyz, lyzl1/2, lalba and lyg precursor genes were transposed to different chromosome regions. The homologue of the mammalian lactalbumin (LALBA) gene was identified for the first time in teleosts and was expressed in skin and during egg and larval development. Lysozyme activity was detected in teleost eggs and varied between species and in the gilthead sea bream lyg and lalba transcript abundance differed in eggs and larvae from different brood stock suggesting differences exist in maternal innate immune protection.



中文翻译:

鱼类溶菌酶基因家族在早期发育中的进化和不同功能。

溶菌酶是先天免疫系统的一组古老的抗菌酶。在这里,我们对鱼类这一最物种的脊椎动物群体早期发育过程中溶菌酶的进化和功能进行了比较分析。在鱼类中,发生了 C 型(鸡或常规)和 G 型(鹅型)基因的谱系和物种特异性进化。系统发育分析表明,硬骨鱼溶菌酶G型成员与四足类同源物同源,而硬骨鱼C型与四足类动物形成三个不同的簇。大多数硬骨鱼C型簇与四足Lyz同群,但也有一些与哺乳动物Lyzl1/2和LALBA同群。这表明在 gnathostome 进化的早期,这些基因已经存在,而lyzl1/2lalba基因存在于鱼和四足动物中。确认序列正交的基因同线性分析未能识别硬骨鱼和其他脊椎动物溶菌酶基因区域之间的保守基因组区域,这表明在祖先硬骨鱼基因组中lyzlyzl1/2lalbalyg前体基因被转座到不同的染色体区域。哺乳动物乳清蛋白 ( LALBA ) 基因的同源物首次在硬骨鱼中发现,并在皮肤以及卵和幼虫发育过程中表达。在硬骨鱼卵中检测到溶菌酶活性,并且在物种之间以及在金头鲷lyglalba中存在差异 来自不同育雏种群的卵和幼虫的转录本丰度不同,这表明母体先天免疫保护存在差异。

更新日期:2020-08-19
down
wechat
bug