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Association of serum C-reactive protein level and polymorphisms with susceptibility to dengue infection and severe clinical outcome among eastern Indian patients.
Medical Microbiology and Immunology ( IF 5.4 ) Pub Date : 2020-07-27 , DOI: 10.1007/s00430-020-00690-2
Saikat Mukherjee 1 , Anusri Tripathi 1
Affiliation  

Dengue virus (DENV) infection is a major public health concern in India ranging from simple febrile illness to severe outcome. This study aimed to investigate association of serum CRP level and CRP gene polymorphisms towards development of dengue disease susceptibility and severity among eastern Indian patients. Blood was collected from 348 symptomatic patients. Sera was subjected to serological diagnosis for the presence of anti-dengue IgM, anti-dengue IgG antibodies and dengue NS1 antigen by ELISA. Viral RNA was extracted and the presence of DENV genome, viral load, serotypes was determined by qRT-PCR. CRP level and polymorphisms were determined by immunoturbidimetry and polymerase chain reaction–restriction fragment length polymorphism, respectively. Statistical analysis was performed by GraphPad-Prism. Among 206 dengue patients, CRP level increased significantly among patients within acute phase, and patients with qRT-PCR/NS1 antigen positivity, high viral load (HVL), secondary infection, and DENV4 and DENV2 infections. rs3091244, TT genotype positively associated with dengue susceptibility (p = 0.03). CT genotype of rs3093059 and TT genotype of rs3091244 were found to correlate with elevated CRP level and development of WHO-defined warning signs. TT genotype of rs3091244 was more prevalent among HVL patients. Thus, these CRP polymorphic variants and CRP concentration might act as potential prognostic biomarkers for predicting disease severity among acute-stage dengue patients.

中文翻译:

印度东部患者血清C反应蛋白水平和多态性与登革热易感性和严重临床结局的关系。

从简单的高热病到严重的结局,登革热病毒(DENV)感染是印度主要的公共卫生问题。这项研究旨在调查印度东部患者中血清CRP水平和CRP基因多态性与登革热疾病易感性和严重性的关系。从348名有症状的患者中收集血液。通过ELISA对血清进行抗登革热IgM,抗登革热IgG抗体和登革热NS1抗原的血清学诊断。提取病毒RNA,并通过qRT-PCR确定DENV基因组的存在,病毒载量,血清型。CRP水平和多态性分别通过免疫比浊法和聚合酶链反应-限制性片段长度多态性确定。通过GraphPad-Prism进行统计分析。在206名登革热患者中,在急性期以及qRT-PCR / NS1抗原阳性,高病毒载量(HVL),继发感染以及DENV4和DENV2感染的患者中,CRP水平显着增加。rs3091244,TT基因型与登革热易感性正相关(p  = 0.03)。发现rs3093059的CT基因型和rs3091244的TT基因型与CRP水平升高和WHO定义的警告信号的发展有关。rs3091244的TT基因型在HVL患者中更为普遍。因此,这些CRP多态性变异体和CRP浓度可能作为预测急性登革热患者疾病严重程度的潜在预后生物标志物。
更新日期:2020-07-27
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