This study evaluated the effect of adding a new step, termed conditioning, to the traditional processing of leaves from Morus alba var. zolwinska wielkolistna grown in Poland (WML-P). This step, modeled on tea leaves processing, was conducted in a controlled environment on a semi-technical scale. The primary goal was to evaluate the effect of the WML-P conditioning for 1–4 h at 32–35 °C on the content of bioactive compounds (total phenolics, phenolic acids, flavonols, 1-deoxynojirimycin) and antioxidant activity (radical scavenging against DPPH, antioxidant capacity, chelating activity and ferric reducing antioxidant potential) of the lyophilized extracts. For the first time WML-P extracts content was comprehensively characterized by assessing dietary fiber fractions, fatty acids, amino acids, macro- and microelements and chlorophyll content. Compared to the traditional process, adding the conditioning step to WML-P processing resulted in an increased total phenolics content, radical scavenging capacity, ability to quench 2,2-diphenyl-1-picrylhydrazyl (DPPH^·) and iron-chelating ability in the lyophilized extracts. The beneficial effect depended on conditioning time. The highest flavonols and phenolic acids content were found after 2-h conditioning. We concluded that adding a 2-h conditioning step to traditional WML-P processing results in getting WML-P lyophilized extract with increased bioactive compounds content and high antioxidant activity.

中文翻译：

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桑树的功能特性和抗氧化活性。Zolwinska Wielkolistna（WML-P）-受控调理过程的影响。

这项研究评估增加一个新台阶的效果，称为调理，叶子从传统的加工桑白皮变种 波兰生长的zolwinska wielkolistna（WML-P）。以茶叶加工为模型的这一步骤是在受控环境中以半技术规模进行的。主要目标是评估在32–35°C下WML-P调理1–4小时对生物活性化合物（总酚，酚酸，黄酮醇，1-脱氧野oji霉素）含量和抗氧化活性（自由基清除）的影响冻干提取物的抗DPPH，抗氧化能力，螯合活性和三价铁还原抗氧化能力）。WML-P提取物的含量首次通过评估膳食纤维级分，脂肪酸，氨基酸，大量和微量元素以及叶绿素含量而得到全面表征。与传统工艺相比，在WML-P工艺中添加调节步骤会导致总酚含量的增加，^·）和冻干提取物中的铁螯合能力。有益效果取决于调节时间。2 h调理后发现最高的黄酮醇和酚酸含量。我们得出的结论是，在传统的WML-P加工过程中添加2小时的调理步骤可得到具有增加的生物活性化合物含量和高抗氧化活性的WML-P冻干提取物。