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Frog Skin-Derived Peptides Against Corynebacterium jeikeium: Correlation between Antibacterial and Cytotoxic Activities.
Antibiotics ( IF 4.8 ) Pub Date : 2020-07-26 , DOI: 10.3390/antibiotics9080448
Bruno Casciaro 1 , Maria Rosa Loffredo 2 , Floriana Cappiello 2 , Walter Verrusio 3 , Vito Domenico Corleto 4 , Maria Luisa Mangoni 2
Affiliation  

Corynebacterium jeikeium is a commensal bacterium that colonizes human skin, and it is part of the normal bacterial flora. In non-risk subjects, it can be the cause of bad body smell due to the generation of volatile odorous metabolites, especially in the wet parts of the body that this bacterium often colonizes (i.e., groin and axillary regions). Importantly, in the last few decades, there have been increasing cases of serious infections provoked by this bacterium, especially in immunocompromised or hospitalized patients who have undergone installation of prostheses or catheters. The ease in developing resistance to commonly-used antibiotics (i.e., glycopeptides) has made the search for new antimicrobial compounds of clinical importance. Here, for the first time, we characterize the antimicrobial activity of some selected frog skin-derived antimicrobial peptides (AMPs) against C. jeikeium by determining their minimum inhibitory and bactericidal concentrations (MIC and MBC) by a microdilution method. The results highlight esculentin-1b(1-18) [Esc(1-18)] and esculentin-1a(1-21) [Esc(1-21)] as the most active AMPs with MIC and MBC of 4–8 and 0.125–0.25 µM, respectively, along with a non-toxic profile after a short- and long-term (40 min and 24 h) treatment of mammalian cells. Overall, these findings indicate the high potentiality of Esc(1-18) and Esc(1-21) as (i) alternative antimicrobials against C. jeikeium infections and/or as (ii) additives in cosmetic products (creams, deodorants) to reduce the production of bad body odor.

中文翻译:

青蛙皮肤衍生的肽抗杆状杆菌:抗菌和细胞毒活性之间的相关性。

杰氏棒状杆菌是在人类皮肤上定居的常见细菌,它是正常细菌菌群的一部分。在非风险受试者中,由于挥发性挥发性代谢物的产生,尤其是在该细菌经常定居的身体湿润部位(例如,腹股沟和腋窝区),可能是造成难闻的身体气味的原因。重要的是,在最近的几十年中,这种细菌引起了严重感染的病例增加,特别是在免疫力下降或住院的患者中,这些患者接受了假体或导管的安装。对常用抗生素(即糖肽)产生耐药性的简便性使得人们在寻找具有临床重要性的新型抗菌化合物。在这里,第一次C. jeikeium通过用微量稀释法测定其最低抑菌和杀菌浓度(MIC和MBC)。结果突出显示esculentin-1b(1-18)[Esc(1-18)]和esculentin-1a(1-21)[Esc(1-21)]是最活跃的AMP,MIC和MBC为4-8,并且短期和长期(40分钟和24小时)处理哺乳动物细胞后,其毒性分别为0.125–0.25 µM和无毒曲线。总体而言,这些发现表明,Esc(1-18)和Esc(1-21)具有以下潜力:(i)替代抗霉菌感染的抗菌剂和/或(ii)化妆品中的添加剂(乳霜,除臭剂)减少恶臭的产生。
更新日期:2020-07-26
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