Staphylococci from Sheedal of Northeast India was isolated, identified and characterized. All the isolated staphylococci were found to be coagulase negative. Based on the rpoB gene sequences followed by analysis using NCBI‐BLAST software, seven species of Staphylococcus namely, S. piscifermentans, S. condimenti, S. arlettae, S. sciuri, S. warneri, S. nepalensis and S. hominis were recognized. Phylogenetic analyses revealed three major cluster groups. All the seven Staphylococcus showed their NaCl tolerance from 2 to 8%. No species was able to grow at 55°C. Except S. arlettae and S. sciuri, all the isolated staphylococcal species exhibited growth at pH 4–8. No isolated species was able to ferment mannitol, sucrose and arabinose. All the species exhibited moderate to maximum proteolytic and lipolytic activities. All the seven species were found to be sensitive to the antibiotics, namely, erythromycin, norfloxacin, ampicillin, streptomycin and vancomycin, whereas all were resistant to co‐trimoxazole. Only S. piscifermentans was found antagonist to Salmonella enterica, Escherichia coli and Bacillus subtilis, although the clear zone was minimal. All the staphylococcal species except S. arlettae and S. sciuri exhibited hydrophobicity ranging from 25 to 66%. The observed characteristics of isolated Staphylococci from Sheedal revealed their role in fish fermentation.

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葡萄球菌属的分离、鉴定和表征。来自印度民族发酵鱼产品

来自印度东北部 Sheedal 的葡萄球菌被分离、鉴定和表征。发现所有分离的葡萄球菌都是凝固酶阴性的。基于 rpoB 基因序列，然后使用 NCBI-BLAST 软件进行分析，识别出 7 种葡萄球菌，即 S. piscifermentans、S. condimenti、S. arlettae、S. sciuri、S. warneri、S. nepalensis 和 S. hominis . 系统发育分析揭示了三个主要的簇群。所有七种葡萄球菌都显示出 2% 至 8% 的 NaCl 耐受性。没有物种能够在 55°C 下生长。除 S. arlettae 和 S. sciuri 外，所有分离的葡萄球菌物种都在 pH 4-8 下生长。没有孤立的物种能够发酵甘露醇、蔗糖和阿拉伯糖。所有物种都表现出中等至最大的蛋白水解和脂肪分解活性。发现所有 7 个物种都对抗生素敏感，即红霉素、诺氟沙星、氨苄青霉素、链霉素和万古霉素，而对复方新诺明耐药。仅发现 S. piscifermentans 是肠道沙门氏菌、大肠杆菌和枯草芽孢杆菌的拮抗剂，尽管透明区域很小。除了 S. arlettae 和 S. sciuri 之外的所有葡萄球菌物种都表现出 25% 到 66% 的疏水性。从 Sheedal 中分离出的葡萄球菌的观察特征揭示了它们在鱼发酵中的作用。除了 S. arlettae 和 S. sciuri 之外的所有葡萄球菌物种都表现出 25% 到 66% 的疏水性。从 Sheedal 中分离出的葡萄球菌的观察特征揭示了它们在鱼发酵中的作用。除了 S. arlettae 和 S. sciuri 之外的所有葡萄球菌物种都表现出 25% 到 66% 的疏水性。从 Sheedal 中分离出的葡萄球菌的观察特征揭示了它们在鱼发酵中的作用。