The fermentation product of Cordyceps sinensis mycelium Cs‐4 was commonly used as alternative substitutes of natural C. sinensis. Massoia lactone is the dominant component in the volatile oil of Cs‐4 mycelium. In this research, we present a high performance liquid chromatography (HPLC) method for the quantitation of massoia lactone in Cs‐4 mycelium. The high and stable contents of massoia lactone with values of 2.98–3.77 mg/g, indicated that massoia lactone could be considered as a marker for the quality assessment of this product. The results of MTT and CCK‐8 assay showed that Cs‐4 mycelium volatiles exhibited cytotoxicity against eight malignant tumor cells (IC₅₀ = 6.0–49.8 μg/ml) in comparison to the anticancer drug 5‐fluorouracil (IC₅₀ = 17.0–425.3 μg/ml), and massoia lactone might be the chemical basis for the anticancer effects of Cs‐4 mycelium. Compared to the commercial drugs paclitaxel and docetaxel (IC₅₀ = 253–1973 μg/ml), the Cs‐4 mycelium volatiles and massoia lactone were discovered to possess inhibitory to taxol‐resistant cell lines (IC₅₀ = 1.5–8.6 μg/ml).

中文翻译：

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高效液相色谱法测定冬虫夏草菌丝体Cs-4中马尾内酯的含量及其体外抗癌活性。

的发酵产物冬虫夏草菌丝铯4通常用作天然的替代品替代冬虫夏草。马尾草内酯是Cs-4菌丝体挥发油中的主要成分。在这项研究中，我们提出了一种高效液相色谱（HPLC）方法，用于定量Cs-4菌丝体中的马尾内酯。马尾草内酯的含量高且稳定，值为2.98–3.77 mg / g，表明马尾草内酯可被视为该产品质量评估的标志。MTT和CCK-8分析的结果表明， 与抗癌药物5-氟尿嘧啶（IC ₅₀）相比，Cs-4菌丝体挥发物对八种恶性肿瘤细胞表现出细胞毒性（IC ₅₀ = 6.0–49.8μg/ ml）。 = 17.0–425.3μg/ ml），马尾内酯可能是Cs-4菌丝体抗癌作用的化学基础。与商业药物紫杉醇和多西他赛相比（IC ₅₀  = 253–1973μg/ ml），发现Cs–4菌丝体挥发物和马尾内酯对紫杉醇耐药细胞系具有抑制作用（IC ₅₀  = 1.5–8.6μg/ ml）。 ）。