Resveratrol (Res) has an anti-tumor effect. Notch signaling components from breast cancer cells treated in culture with Resveratrol was investigated. MDA-MB-231cells were divided into control group (Res-untreated) and Res-treated groups including six concentrations 0 μM, 10 μM, 20 μM, 40 μM, 80 μM and 160 μM. Cytotoxicity test were evaluated by CCK-8. The mRNA and Protein expression levels of Notch1, Jagged1, Dll4 and Hes-5 were detected by RT-PCR and Western blot. The mRNA expression of Notch1, Jagged1, Hes-5 and Dll4 in the Res administration group decreased significantly (0.01 < p < .05), and Hes-5 and Dll4 were extremely significant (p < .01). Compared with the blank control group, the protein expression of Notch1 and Dll4 decreased significantly in each concentration Res group, but the decrease of protein expression of Jagged1 and Hes-5 was not significant. In conclusion, Res regulates mRNA and protein expression of Notch1, Dll4 of MDA-MB-231 cells via Notch pathway.

白藜芦醇（Res）具有抗肿瘤作用。研究了用白藜芦醇处理的乳腺癌细胞中的Notch信号成分。将MDA-MB-231细胞分为对照组（未经Res处理的）和经过Res-处理的组，包括六个浓度为0μM，10μM，20μM，40μM，80μM和160μM的浓度。通过CCK-8评价细胞毒性试验。RT-PCR和Western blot检测Notch1，Jagged1，Dll4和Hes-5的mRNA和蛋白表达水平。Res给药组中Notch1，Jagged1，Hes-5和Dll4的mRNA表达显着下降（0.01 <  p  <.05），而Hes-5和Dll4的异常表达（p <.01）。与空白对照组相比，每个浓度Res组Notch1和Dll4的蛋白表达均显着下降，但Jagged1和Hes-5的蛋白表达下降不明显。总之，Res通过Notch途径调节MDA-MB-231细胞Notch1，Dll4的mRNA和蛋白表达。